UAM_Biblioteca | Unified search engine | Scientific Production Portal | UAM Research Data Repository
Universidad Autónoma de Madrid
Biblos-e Archivo
We are onFacebookCanal BiblosYouTubeTwitterPinterestWhatsappInstagram
    • español
    • English
  • English 
    • español
    • English
  • Log in
JavaScript is disabled for your browser. Some features of this site may not work without it.

Search Biblos-e Archivo

Advanced Search

Browse

All of Biblos-e ArchivoCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsFacultiesThis CollectionBy Issue DateAuthorsTitlesSubjectsFaculties

My Account

Log inRegister

Statistics

View Usage Statistics

Help

Information about Biblos-e ArchivoI want to submit my workI want to submit my doctoral thesisFrequently Asked QuestionsCopyrightsFinancial Agencies and OA policy

UAM_Biblioteca

View Item 
  •   Biblos-e Archivo
  • Producción científica en acceso abierto de la UAM
  • Producción científica en acceso abierto de la UAM
  • View Item
  •   Biblos-e Archivo
  • Producción científica en acceso abierto de la UAM
  • Producción científica en acceso abierto de la UAM
  • View Item

Adipose tissue promotes a serum cytokine profile related to lower insulin sensitivity after chronic central leptin infusion

Author
Burgos-Ramos, Emma; Canelles, Sandra; Perianes-Cachero, Arancha; Arilla-Ferreiro, Eduardo; Argente, Jesús; Barrios, Vicente
Entity
UAM. Departamento de Pediatría
Publisher
Public Library of Science
Date
2012-10-02
Citation
10.1371/journal.pone.0046893
Plos One 7.10 (2012): e46893
 
 
 
ISSN
1932-6203 (online)
DOI
10.1371/journal.pone.0046893
Funded by
This work was supported by grants from Fondo de Investigación Sanitaria (PI10/0747), Ministerio de Ciencia y Tecnología (SAF 2010-22277), CIBERobn (CB03/06) and Fundación Endocrinología y Nutrición. S.C. is supported by CIBERobn
Project
Gobierno de España. PI10/0747; Gobierno de España. SAF 2010-22277
Subjects
Cytokines; Homeostasis; Infusion Pumps; Insulin Resistance; Intra-Abdominal Fat; Signal Transduction; Medicina
URI
http://hdl.handle.net/10486/665701
Rights
© 2012 Burgos-Ramos et al.

Licencia Creative Commons
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.

Abstract

Obesity is an inflammatory state characterized by an augment in circulating inflammatory factors. Leptin may modulate the synthesis of these factors by white adipose tissue decreasing insulin sensitivity. We have examined the effect of chronic central administration of leptin on circulating levels of cytokines and the possible relationship with cytokine expression and protein content as well as with leptin and insulin signaling in subcutaneous and visceral adipose tissues. In addition, we analyzed the possible correlation between circulating levels of cytokines and peripheral insulin resistance. We studied 18 male Wistar rats divided into controls (C), those treated icv for 14 days with a daily dose of 12 μg of leptin (L) and a pair-fed group (PF) that received the same food amount consumed by the leptin group. Serum leptin and insulin were measured by ELISA, mRNA levels of interferon-γ (IFN-γ), interleukin-2 (IL-2), IL-4, IL-6, IL-10 and tumor necrosis factor-α (TNF-α) by real time PCR and serum and adipose tissue levels of these cytokines by multiplexed bead immunoassay. Serum leptin, IL-2, IL-4, IFN-γ and HOMA-IR were increased in L and TNF-α was decreased in PF and L. Serum leptin and IL-2 levels correlate positively with HOMA-IR index and negatively with serum glucose levels during an ip insulin tolerance test. In L, an increase in mRNA levels of IL-2 was found in both adipose depots and IFN-γ only in visceral tissue. Activation of leptin signaling was increased and insulin signaling decreased in subcutaneous fat of L. In conclusion, leptin mediates the production of inflammatory cytokines by adipose tissue independent of its effects on food intake, decreasing insulin sensitivity
Show full item record

Files in this item

Thumbnail
Name
adipose_burgos_PO_2012.pdf
Size
600.3Kb
Format
PDF

Refworks Export

Delicious Save this on Delicious

Google™ Scholar:Burgos-Ramos, Emma - Canelles, Sandra - Perianes-Cachero, Arancha - Arilla-Ferreiro, Eduardo - Argente, Jesús - Barrios, Vicente

This item appears in the following Collection(s)

  • Producción científica en acceso abierto de la UAM [14411]

Related items

Showing items related by title, author, creator and subject.

  • Improvement in glycemia after glucose or insulin overload in leptin-infused rats is associated with insulin-related activation of hepatic glucose metabolism 

    Burgos-Ramos, Emma; Canelles, Sandra; Frago. Laura M.; Chowen, Julie Ann; Arilla-Ferreiro, Eduardo; Argente, Jesús; Barrios, Vicente
    2016-03-01
  • Cerebral insulin bolus revokes the changes in hepatic lipid metabolism induced by chronic central leptin infusion 

    Barrios, Vicente; López-Villar, Elena; Frago, Laura M.; Canelles, Sandra; Díaz-González, Francisca; Burgos-Ramos, Emma; Frühbeck, Gema; Chowen, Julie A.; Argente, Jesús
    2021-03-06
  • Leptin modulates the response of brown adipose tissue to negative energy balance: implication of the gh/igf-i axis 

    Barrios, Vicente; Frago, Laura M.; Canelles, Sandra; Guerra-Cantera, Santiago; Arilla-Ferreiro, Eduardo; Chowen, Julie A.; Argente, Jesús
    2021-03-11
All the documents from Biblos-e Archivo are protected by copyrights. Some rights reserved.
Universidad Autónoma de Madrid. Biblioteca
Contact Us | Send Feedback
We are onFacebookCanal BiblosYouTubeTwitterPinterestWhatsappInstagram
 

 

All the documents from Biblos-e Archivo are protected by copyrights. Some rights reserved.
Universidad Autónoma de Madrid. Biblioteca
Contact Us | Send Feedback
We are onFacebookCanal BiblosYouTubeTwitterPinterestWhatsappInstagram