Characterization In vitro and In vivo of a pandemic H1N1 influenza virus from a fatal case
Entity
UAM. Departamento de Medicina Preventiva y Salud Pública y MicrobiologíaPublisher
Public Library of ScienceDate
2013-01-17Citation
10.1371/journal.pone.0053515
Plos One 8.1 (2013): e53515
ISSN
1932-6203 (online)DOI
10.1371/journal.pone.0053515Funded by
This work was supported by Instituto de Salud Carlos III (Programa especial de investigación sobre la gripe pándemica GR09/0023, GR09/0040, GR09/ 0039) and Ciber de Enfermedades RespiratoriasSubjects
Amino Acids; Cells; Immunohistochemistry; Influenza; Pandemics; Inbred BALB C; MedicinaRights
© 2013 Rodriguez et al.Abstract
Pandemic 2009 H1N1 (pH1N1) influenza viruses caused mild symptoms in most infected patients. However, a greater rate of severe disease was observed in healthy young adults and children without co-morbid conditions. Here we tested whether influenza strains displaying differential virulence could be present among circulating pH1N1 viruses. The biological properties and the genotype of viruses isolated from a patient showing mild disease (M) or from a fatal case (F), both without known co-morbid conditions were compared in vitro and in vivo. The F virus presented faster growth kinetics and stronger induction of cytokines than M virus in human alveolar lung epithelial cells. In the murine model in vivo, the F virus showed a stronger morbidity and mortality than M virus. Remarkably, a higher proportion of mice presenting infectious virus in the hearts, was found in F virus-infected animals. Altogether, the data indicate that strains of pH1N1 virus with enhanced pathogenicity circulated during the 2009 pandemic. In addition, examination of chemokine receptor 5 (CCR5) genotype, recently reported as involved in severe influenza virus disease, revealed that the F virus-infected patient was homozygous for the deleted form of CCR5 receptor (CCR5Δ32)
Files in this item
Google Scholar:Rodriguez, Ariel
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Falcón, Ana María
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Cuevas, María Teresa
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Pozo, Francisco
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Guerra, Susana
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García-Barreno, Blanca
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Martínez-Orellana, Pamela
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Pérez-Breña, Pilar
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Montoya, María
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Melero, José Antonio
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Pizarro, Manuel
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Ortín, Juan
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Casas, Inmaculada
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Nieto, Amelia
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