Show simple item record

dc.contributor.authorArroyo-Villa, Irene
dc.contributor.authorBautista-Caro, María Belén
dc.contributor.authorBalsa Criado, Alejandro 
dc.contributor.authorAguado Acín, Pilar
dc.contributor.authorNuño, Laura
dc.contributor.authorBonilla-Hernán, María Gema
dc.contributor.authorPuig-Kröger, Amaya
dc.contributor.authorMartìn-Mola, Emilio
dc.contributor.authorMiranda-Carús, María Eugenia
dc.contributor.otherUAM. Departamento de Medicinaes_ES
dc.date.accessioned2015-06-18T10:08:38Z
dc.date.available2015-06-18T10:08:38Z
dc.date.issued2012-08-03
dc.identifier.citationPlos One 7.8 (2012): e42189en_US
dc.identifier.issn1932-6203 (online)en_US
dc.identifier.urihttp://hdl.handle.net/10486/666902
dc.description.abstractObjective: To examine the frequency and phenotype of Th17 cells in the peripheral blood of early RA (eRA) patients. Methods: CD4+ T cells were isolated from the peripheral blood of 33 eRA patients, 20 established RA patients and 53 healthy controls (HC), and from the synovial fluid of 20 established RA patients (RASF), by ficoll-hypaque gradient and magnetical negative selection. After polyclonal stimulation, the frequency of Th17 and Th1 cells was determined by flow cytometry and concentrations of IL-17, IFN-γ, TNF-α and IL-10 were measured by ELISA in cell-free supernatants. Results: When all of our eRA patients were analyzed together, a significantly lower percentage of circulating Th17 cells and a lower CD4-derived IL-17 secretion were observed in comparison with HC. However, after stratifying by anti-CCP antibody status, circulating Th17 cells were decreased in anti-CCP(+) but not in anti-CCP(-)-eRA. All Th17 cells were CD45RO+CD45RA- and CCR6+. Dual Th17/Th1 cells were also exclusively decreased in anti-CCP(+)-eRA. Circulating Th17 and Th17/Th1 cells were negatively correlated with anti-CCP titres. When anti-CCP(+)-eRA patients were retested one year after initiating treatment with oral methotrexate, their circulating Th17 frequency was no longer different from HC. Of note, the percentage of circulating Th1 cells and the secretion of CD4-derived IFN-γ, TNF-α and IL-10 were not different between eRA patients and HC. In established RA patients, circulating Th17 and T17/Th1 cell frequencies were comparable to HC. In RASF, both Th17 and Th1 cells were increased when compared with blood of eRA patients, established RA patients and HC. Conclusion: Decreased circulating Th17 levels in eRA seem to be a marker of anti-CCP seropositivity, and return to levels observed in healthy controls after treatment with methotrexateen_US
dc.description.sponsorshipThis work was supported by Ministerio de Ciencia e Innovación grant SAF 2009-07100, (http://www.idi.mineco.gob.es/portal/site/MICINN) and by RETICS Program, RD08/0075 (RIER) from ‘‘Instituto de Salud Carlos III’’ (ISCIII) (http://www.isciii.es/)en_US
dc.format.extent8 pag.es_ES
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPlos Oneen_US
dc.rights© 2012 Arroyo-Villa et al.es_ES
dc.subject.otherArthritisen_US
dc.subject.otherRheumatoiden_US
dc.subject.otherAgeden_US
dc.subject.otherBiological Markersen_US
dc.subject.otherSynovial Fluiden_US
dc.subject.otherCytokinesen_US
dc.subject.otherTh17 Cellsen_US
dc.titleFrequency of Th17 CD4+ T cells in early rheumatoid arthritis: A marker of anti-CCP seropositivityen_US
dc.typearticleen
dc.subject.ecienciaMedicinaes_ES
dc.identifier.doi10.1371/journal.pone.0042189es_ES
dc.identifier.publicationfirstpagee42189es_ES
dc.identifier.publicationissue8es_ES
dc.identifier.publicationlastpagee42189es_ES
dc.identifier.publicationvolume7es_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.rights.ccReconocimientoes_ES
dc.rights.accessRightsopenAccessen
dc.facultadUAMFacultad de Medicina


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record