Monkey adrenal chromaffin cells express α6β4* nicotinic acetylcholine receptors
Entity
UAM. Departamento de FarmacologíaPublisher
Public Library of ScienceDate
2014-04-11Citation
10.1371/journal.pone.0094142
Plos One 9.4 (2014): e94142
ISSN
1932-6203 (online)DOI
10.1371/journal.pone.0094142Funded by
This work was supported by grants from the Ministerio de Ciencia e Innovación No. BFU2011-27690 and BFU2012-30997 awarded to AA and by grants from the U.S. National Institutes of Health GM103801 and GM48677 to JMM. AHV holds a fellowship award from the Universidad Autónoma de Madrid. AJH hold a Marie Curie International Fellowship from the European CommissionSubjects
Chromaffin Cells; Conotoxins; Evoked Potentials; Haplorhini; Polymerase Chain Reaction; Patch-Clamp Techniques; MedicinaRights
© 2014 Hernández-Vivanco et al.Abstract
Nicotinic acetylcholine receptors (nAChRs) that contain α6 and β4 subunits have been demonstrated functionally in human adrenal chromaffin cells, rat dorsal root ganglion neurons, and on noradrenergic terminals in the hippocampus of adolescent mice. In human adrenal chromaffin cells, α6β4* nAChRs (the asterisk denotes the possible presence of additional subunits) are the predominant subtype whereas in rodents, the predominant nAChR is the α3β4* subtype. Here we present molecular and pharmacological evidence that chromaffin cells from monkey (Macaca mulatta) also express α6β4* receptors. PCR was used to show the presence of transcripts for α6 and β4 subunits and pharmacological characterization was performed using patch-clamp electrophysiology in combination with α-conotoxins that target the α6β4* subtype. Acetylcholine-evoked currents were sensitive to inhibition by BuIA[T5A,P6O] and MII[H9A,L15A]; α-conotoxins that inhibit α6-containing nAChRs. Two additional agonists were used to probe for the expression of α7 and β2-containing nAChRs. Cells with currents evoked by acetylcholine were relatively unresponsive to the α7-selctive agonist choline but responded to the agonist 5-I-A-85380. These studies provide further insights into the properties of natively expressed α6β4* nAChRs
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Google Scholar:Hernández-Vivanco, Alicia
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Hone, Arik J.
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Scadden, Mickl
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Carmona-Hidalgo, Beatriz
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McIntosh, J. Michael
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Albillos Martínez, María Almudena
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