Use of echocardiography reveals reestablishment of ventricular pumping efficiency and partial ventricular wall motion recovery upon ventricular cryoinjury in the zebrafish
Entity
UAM. Departamento de MedicinaPublisher
Public Library of ScienceDate
2014-12-22Citation
10.1371/journal.pone.0115604
Plos One 9.12 (2014): e115604
ISSN
1932-6203DOI
10.1371/journal.pone.0115604Funded by
Funding was from the Fundación CNIC Carlos III, the Fundación ProCNIC, the Spanish Ministry of Economy and Competitiveness (Tercel and BFU2011-25297 to N.M., FPU AP2008-00546 to J.M.G.-R. and FPU12/03007 to H.S-I.), the Community of Madrid (FIBROTEAM S2010/BMD- 2321 to N.M), PIEF-GA-2012-330728 to I.J.M. and an ERC Starting grant 337703 – zebraHeart to N.M.Project
Comunidad de Madrid. S2010/BMD-2321/FIBROTEAMEditor's Version
http://dx.doi.org/10.1371/journal.pone.0115604Subjects
2D-echocardiography; Ventricular; MedicinaNote
The authors confirm that all data underlying the findings are fully available without restriction. All relevant data are within the paper and its Supporting Information filesRights
© 2014 González-Rosa et al.Abstract
Aims: While zebrafish embryos are amenable to in vivo imaging, allowing the study
of morphogenetic processes during development, intravital imaging of adults is
hampered by their small size and loss of transparency. The use of adult zebrafish
as a vertebrate model of cardiac disease and regeneration is increasing at high
speed. It is therefore of great importance to establish appropriate and robust
methods to measure cardiac function parameters.
Methods and Results: Here we describe the use of 2D-echocardiography to study
the fractional volume shortening and segmental wall motion of the ventricle. Our
data show that 2D-echocardiography can be used to evaluate cardiac injury and
also to study recovery of cardiac function. Interestingly, our results show that while
global systolic function recovered following cardiac cryoinjury, ventricular wall
motion was only partially restored.
Conclusion: Cryoinjury leads to long-lasting impairment of cardiac contraction,
partially mimicking the consequences of myocardial infarction in humans.
Functional assessment of heart regeneration by echocardiography allows a deeper
understanding of the mechanisms of cardiac regeneration and has the advantage
of being easily transferable to other cardiovascular zebrafish disease models
Files in this item
Google Scholar:González-Rosa, Juan
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Guzmán-Martínez, Gabriela
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Marqués, Inés Joao
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Sánchez-Iranzo, Héctor
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Jiménez-Borreguero, Luis Jesús
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Mercader, Nadia
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