ISG15 Regulates Peritoneal Macrophages Functionality against Viral Infection
Entity
UAM. Departamento de Medicina Preventiva y Salud Pública y MicrobiologíaPublisher
Public Library of ScienceDate
2013-10-01Citation
10.1371/journal.ppat.1003632
PloS Pathogens 9.10 (2013): e1003632
ISSN
1553-7366 (print); 1553-7374 (online)DOI
10.1371/journal.ppat.1003632Funded by
This work was supported by grants from the Spanish Ministry of Health FIS2011-00127, Comunidad de Madrid UAM-CM-CCG10-4911 and UAM-Banco de Santander to SG. This work was also partly supported by NIAID grant U19AI083025 and by CRIP (Center for Research on Influenza Pathogenesis, HHSN266200700010C), a NIAID Center of Excellence for Influenza Research and Surveillance (CEIRS) to AGS. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscriptProject
Gobierno de España. FIS2011-00127Editor's Version
http://dx.doi.org/10.1371/journal.ppat.1003632Subjects
Viral infection; Antiviral; MedicinaRights
© 2013 Yángüez et al.Abstract
Upon viral infection, the production of type I interferon (IFN) and the subsequent upregulation of IFN stimulated genes
(ISGs) generate an antiviral state with an important role in the activation of innate and adaptive host immune responses.
The ubiquitin-like protein (UBL) ISG15 is a critical IFN-induced antiviral molecule that protects against several viral infections,
but the mechanism by which ISG15 exerts its antiviral function is not completely understood. Here, we report that ISG15
plays an important role in the regulation of macrophage responses. ISG152/2 macrophages display reduced activation,
phagocytic capacity and programmed cell death activation in response to vaccinia virus (VACV) infection. Moreover,
peritoneal macrophages from mice lacking ISG15 are neither able to phagocyte infected cells nor to block viral infection in
co-culture experiments with VACV-infected murine embryonic fibroblast (MEFs). This phenotype is independent of cytokine
production and secretion, but clearly correlates with impaired activation of the protein kinase AKT in ISG15 knock-out (KO)
macrophages. Altogether, these results indicate an essential role of ISG15 in the cellular immune antiviral response and
point out that a better understanding of the antiviral responses triggered by ISG15 may lead to the development of
therapies against important human pathogens
Files in this item
Google Scholar:Yángüez, Emilio
-
García-Culebras, Alicia
-
Frau, Aldo
-
Llompart, Catalina
-
Knobeloch, Klaus Peter
-
Gutiérrez-Erlandsson, Sylvia
-
García-Sastre, Adolfo
-
Esteban, Mariano
-
Nieto, Amelia
-
Guerra García, María Susana
This item appears in the following Collection(s)
Related items
Showing items related by title, author, creator and subject.
-
Genomic and ultrastructural analysis of monkeypox virus in skin lesions and in human/animal infected cells reveals further morphofunctional insights into viral pathogenicity
Paniz-Mondolfi, Alberto; Reidy, Jason; Pagani, Nina; Lednicky, John A.; McGrail, Joseph Patrick; Kasminskaya, Yana; Patino, Luz H.; Garcia-Sastre, Adolfo; Palacios, Gustavo; Gonzalez-Reiche, Ana Silvia; van Bakel, Harm; Firpo Betancourt, Adolfo; Hernandez, Matthew M.; Cordon-Cardo, Carlos; Simon, Viviana; Sordillo, Emilia M.; Ramírez, Juan David; Guerra García, María Susana
2023-07-15