Función y regulación de las homeoproteínas del complejo génico Iroquois en los discos imaginales de ala y ojo-antena de Drosophila melanogaster
Author
González Pérez, EstherAdvisor
Campuzano Corrales, SonsolesEntity
UAM. Departamento de Biología MolecularDate
2009-06-19Subjects
Drosophilas - Tesis doctorales; Biología y Biomedicina / BiologíaNote
Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 19-06-2009Abstract
The homeoproteins encoded by the Iroquois complex (Iro-C) genes, Araucan
(Ara), Caupolican (Caup) and Mirror (Mirr), are involved in several processes during the
development of Drosophila melanogaster, according to their expression patterns.
In this Thesis, to gain further insight into the role played by these proteins in
the specification of the mesothorax (or notum) and in the formation of the pattern of
sensory organs, we have focussed in the analysis of the spatial and temporal regulation
of the expression of the Iro-C genes and also in the study of the functional specificity
of the Iro-C proteins.
We have generated novel Iro-C regulatory mutants, which have allowed us
to characterize in vivo the function of Iro-C enhancers previously identified in our
laboratory, to hypothesize the existence of additional enhancers, with suggestion of
their putative localization in the Iro-C, and to support the idea that ara and caup
expression is controlled by shared enhancers. Furthermore, we have identified the
presence in the Iro-C of putative insulator sequences (dCTCF binding sites) that would
help to establish an independent control of ara/caup and mirr expression.
Iro-C proteins show a high degree of similarity in their homeodomain and
accumulate in similar patterns in the presumptive notum region of the wing imaginal
disc. Our data strongly support the role of all the Iro-C proteins as activators of the
expression of the proneural genes of the achaete-scute complex that define the pattern
of external sensory organs. However, there appears to be specific requirements of
certain Iro-C proteins for bristle development, thus suggesting that function of Iro-C
proteins in this process is not completely redundant.
The generation of novel Iro-C regulatory mutants has uncovered a novel function
of Iro-C genes: their involvement in the development of the maxillary palp. It should
be stressed that this function appears to rely in the expression of Iro-C genes in the
peripodial epithelium of the eye-antennal disc.
Files in this item
Google Scholar:González Pérez, Esther
This item appears in the following Collection(s)
Related items
Showing items related by title, author, creator and subject.
-
Competición celular y desarrollo de tumores en discos imaginales de drosophila melanogaster
Menéndez González, Javier
2011 -
Función del gen asense del complejo geníco achaete-scute en la generación de los órganos sensoriales de Drosophila melanogaster
Domínguez Castellano, María
1993-04-02 -
La región haploletal del complejo génico "Shaker de Drosophila melanogaster": estructura y función
Prado Moreno, Antonio
1996-10-07