Función y regulación de las homeoproteínas del complejo génico Iroquois en los discos imaginales de ala y ojo-antena de Drosophila melanogaster
AdvisorCampuzano Corrales, Sonsoles
EntityUAM. Departamento de Biología Molecular
SubjectsDrosophilas - Tesis doctorales; Biología y Biomedicina / Biología
NoteTesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 19-06-2009
The homeoproteins encoded by the Iroquois complex (Iro-C) genes, Araucan (Ara), Caupolican (Caup) and Mirror (Mirr), are involved in several processes during the development of Drosophila melanogaster, according to their expression patterns. In this Thesis, to gain further insight into the role played by these proteins in the specification of the mesothorax (or notum) and in the formation of the pattern of sensory organs, we have focussed in the analysis of the spatial and temporal regulation of the expression of the Iro-C genes and also in the study of the functional specificity of the Iro-C proteins. We have generated novel Iro-C regulatory mutants, which have allowed us to characterize in vivo the function of Iro-C enhancers previously identified in our laboratory, to hypothesize the existence of additional enhancers, with suggestion of their putative localization in the Iro-C, and to support the idea that ara and caup expression is controlled by shared enhancers. Furthermore, we have identified the presence in the Iro-C of putative insulator sequences (dCTCF binding sites) that would help to establish an independent control of ara/caup and mirr expression. Iro-C proteins show a high degree of similarity in their homeodomain and accumulate in similar patterns in the presumptive notum region of the wing imaginal disc. Our data strongly support the role of all the Iro-C proteins as activators of the expression of the proneural genes of the achaete-scute complex that define the pattern of external sensory organs. However, there appears to be specific requirements of certain Iro-C proteins for bristle development, thus suggesting that function of Iro-C proteins in this process is not completely redundant. The generation of novel Iro-C regulatory mutants has uncovered a novel function of Iro-C genes: their involvement in the development of the maxillary palp. It should be stressed that this function appears to rely in the expression of Iro-C genes in the peripodial epithelium of the eye-antennal disc.
Files in this item
Texto de la Tesis Doctoral
Google Scholar:González Pérez, Esther
This item appears in the following Collection(s)
Showing items related by title, author, creator and subject.
Menéndez González, Javier
Función del gen asense del complejo geníco achaete-scute en la generación de los órganos sensoriales de Drosophila melanogaster Domínguez Castellano, María
La región haploletal del complejo génico "Shaker de Drosophila melanogaster": estructura y función Prado Moreno, Antonio