Alterations in perivascular sympathetic and nitrergic innervation function induced by late pregnancy in rat mesenteric arteries
Entity
UAM. Departamento de Fisiología; Instituto de Investigación Sanitaria Hospital Universitario de La Paz (IdiPAZ)Publisher
Public Library of ScienceDate
2015-05-07Citation
10.1371/journal.pone.0126017
PlLos ONE 10.5 (2015): e0126017
ISSN
1932-6203DOI
10.1371/journal.pone.0126017Funded by
This study was supported by Ministerio de Economía y Competitividad (SAF2012-38530), Red RIC (RD12/0042/0024), and Fundación MAPFRE. E. Sastre received a FPI-UAM fellowship. L. Caracuel received a fellowship from Alianza 4 Universidades Program.Project
Gobierno de España. SAF2012-38530Editor's Version
http://dx.doi.org/10.1371/journal.pone.0126017Subjects
Pregnancy; Mesenteric arteries; EFS; MedicinaRights
© 2015 Sastre et al.Abstract
Background and Purpose
We investigated whether pregnancy was associated with changed function in components
of perivascular mesenteric innervation and the mechanism/s involved.
Experimental Approach
We used superior mesenteric arteries from female Sprague-Dawley rats divided into two
groups: control rats (in oestrous phase) and pregnant rats (20 days of pregnancy). Modifications
in the vasoconstrictor response to electrical field stimulation (EFS) were analysed in
the presence/absence of phentolamine (alpha-adrenoceptor antagonist) or L-NAME (nitric
oxide synthase-NOS- non-specific inhibitor). Vasomotor responses to noradrenaline (NA),
and to NO donor DEA-NO were studied, NA and NO release measured and neuronal NOS
(nNOS) expression/activation analysed.
Key Results
EFS induced a lower frequency-dependent contraction in pregnant than in control rats.
Phentolamine decreased EFS-induced vasoconstriction in segments from both experimental
groups, but to a greater extent in control rats. EFS-induced vasoconstriction was increased
by L-NAME in arteries from both experimental groups. This increase was greater in
segments from pregnant rats. Pregnancy decreased NA release while increasing NO release.
nNOS expression was not modified but nNOS activation was increased by pregnancy.
Pregnancy decreased NA-induced vasoconstriction response and did not modify DEANO-
induced vasodilation response
Files in this item
Google Scholar:Sastre, Esther
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Blanco Rivero, Javier
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Caracuel, Laura
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Callejo, María
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Balfagón, Gloria
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