Adipose-derived stem cells and platelet-rich plasma for preventive treatment of bisphosphonate-related osteonecrosis of the jaw in a murine model
Entity
UAM. Departamento de CirugíaPublisher
Elsevier Inc.Date
2015-09-01Citation
10.1016/j.jcms.2015.04.026
Journal of Cranio-Maxillofacial Surgery 43.7 (2015): 1161-1168
ISSN
1010-5182DOI
10.1016/j.jcms.2015.04.026Funded by
This study was supported by a grant from the Spanish Ministry of Health and Consumer Affairs (FIS PI10/01991) and a RETICS grant from ISCIII (RD12/0019/0035) and the Education Council of Madrid (P2010/BMD-2420).Project
Gobierno de España. FIS PI10/01991; Comunidad de Madrid. S2010/BMD-2420/CELLCAMEditor's Version
http://dx.doi.org/10.1016/j.jcms.2015.04.026Subjects
Adipose-derived stem cells; Platelet-rich plasma; Bisphosphonate-related osteonecrosis of the jaw; Zoledronic acid; MedicinaNote
This is the author’s version of a work that was accepted for publication in Journal of Cranio-Maxillofacial Surgery. A definitive version was subsequently published in Journal of Cranio-Maxillofacial Surgery 43.7 (2015) DOI: 10.1016/j.jcms.2015.04.026Abstract
Objectives: The main challenge in treating bisphosphonate-related osteonecrosis of the jaw (BRONJ) is
the absence of an effective established treatment. We aimed to compare different potentially preventive
treatments for BRONJ after dental extractions in zoledronic acid (ZA)-treated animals. We studied the
local application of different combinations of adipose-derived stem cells (ASCs) with or without previous
stimulation with bone morphogenetic protein 2 (BMP-2) and platelet-rich plasma (PRP) in rats.
Material and methods: Fifty-six male Wistar rats were treated with ZA for 9 weeks. Dental extractions
were performed in the eighth week, and the animals were divided into 4 groups. In group 1 (n ¼ 14),
alveolar coverage with mucoperiosteal flap was performed. In group 2 (n ¼ 14), PRP was applied over the
sockets and covered with the flap. In group 3 (n ¼ 15), allogeneic ASCs with PRP were applied and
covered with the flap. In group 4 (n ¼ 13), animals were treated with ASCs cultured with BMP-2, PRP, and
flap coverage. Histologic, fluorescence, and radiologic studies of the maxillae were performed.
Results: ASC-treated animals showed lower frequency of osteonecrosis (14% vs 50%, p ¼ 0.007) and
greater bone turnover (p ¼ 0.024) and osteoclast count (p ¼ 0.045) than those not receiving the ASC
treatment.
Conclusions: In this high-risk model, ASC-based treatments seem to prevent BRONJ more effectively than
mucosal flap with or without PRP. The combination of ASCs and PRP appears to be synergistic, and the
addition of BMP-2 could further improve the results
Files in this item
Google Scholar:Barba-Recreo, Paula
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Castillo Pardo De Vera, Jose Luis del
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Georgiev-Hristov, Tihomir
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Ruiz Bravo-Burguillos, Elena
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Abarrategi, Ander
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Burgueño, Miguel
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García Arranz, Mariano Andrés
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