Galactomannan downregulates the inflammation responses in human macrophages via NF κ B2/p100

Abstract

We show that galactomannan, a polysaccharide consisting of amannose backbone with galactose side groups present on the cell wall of several fungi, induces a reprogramming of the inflammatory response in human macrophages through dectin-1 receptor. The nuclear factor kappa-light-chain-enhancer of activated B cells 2 (NF𝜅B2)/p100 was overexpressed after galactomannan challenge. Knocking down NF𝜅B2/p100 using small interfering RNA (siRNA) indicated that NF𝜅B2/p100 expression is a crucial factor in the progression of the galactomannan-induced refractoriness. The data presented in this study could be used as a modulator of inflammatory response in clinical situations where refractory state is required.