dc.contributor.author | Quer, Josep | |
dc.contributor.author | Gregori, Josep | |
dc.contributor.author | Rodríguez-Frias, Francisco | |
dc.contributor.author | Buti, Maria | |
dc.contributor.author | Madejon, Antonio | |
dc.contributor.author | Perez-del-Pulgar, Sofia | |
dc.contributor.author | Garcia-Cehic, Damir | |
dc.contributor.author | Casillas, Rosario | |
dc.contributor.author | Blasi, Maria | |
dc.contributor.author | Homs, Maria | |
dc.contributor.author | Tabernero, David | |
dc.contributor.author | Alvarez-Tejado, Miguel | |
dc.contributor.author | Muñoz, Jose Manuel | |
dc.contributor.author | Cubero, Maria | |
dc.contributor.author | Caballero, Andrea | |
dc.contributor.author | Campo, Jose Antonio del | |
dc.contributor.author | Domingo, Esteban | |
dc.contributor.author | Belmonte, Irene | |
dc.contributor.author | Nieto, Leonardo | |
dc.contributor.author | Lens, Sabela | |
dc.contributor.author | Muñoz-de-Rueda, Paloma | |
dc.contributor.author | Sanz-Cameno, Paloma | |
dc.contributor.author | Sauleda, Silvia | |
dc.contributor.author | Bes, Marta | |
dc.contributor.author | Gomez, Jordi | |
dc.contributor.author | Briones, Carlos | |
dc.contributor.author | Perales, Celia | |
dc.contributor.author | Sheldon, Julie | |
dc.contributor.author | Castells, Lluis | |
dc.contributor.author | Viladomiu, Lluis | |
dc.contributor.author | Salmerón, Javier | |
dc.contributor.author | Ruiz-Extremera, Angela | |
dc.contributor.author | Quiles-Pérez, Rosa | |
dc.contributor.author | Moreno-Otero, Ricardo | |
dc.contributor.author | López-Rodríguez, Rosario | |
dc.contributor.author | Allende, Helena | |
dc.contributor.author | Romero-Gómez, Manuel | |
dc.contributor.author | Guardia, Jaume | |
dc.contributor.author | Esteban, Rafael | |
dc.contributor.author | Garcia-Samaniego, Javier | |
dc.contributor.author | Forns, Xavier | |
dc.contributor.author | Esteban, Juan Ignacio | |
dc.contributor.other | UAM. Departamento de Medicina | es_ES |
dc.contributor.other | Centro de Biología Molecular Severo Ochoa (CBM) | es_ES |
dc.date.accessioned | 2016-07-20T14:46:07Z | |
dc.date.available | 2016-07-20T14:46:07Z | |
dc.date.issued | 2015-01-01 | |
dc.identifier.citation | Journal of Clinical Microbiology 53.1 (2015): 219-226 | es_ES |
dc.identifier.issn | 0095-1137 | es_ES |
dc.identifier.issn | 1098-660X (on line) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10486/672182 | |
dc.description.abstract | HepatitisCvirus(HCV)is classified into seven major genotypesand67 subtypes. Recent studies haveshownthat inHCVgenotype 1-infected
patients, response rates to regimens containingdirect-acting antivirals(DAAs)are subtype dependent. Currently available genotypingmethods
have limited subtyping accuracy.Wehave evaluated theperformanceof adeep-sequencing-basedHCVsubtyping assay, developed for the
454/GS-Junior platform, in comparisonwith thoseof two commercial assays (VersantHCVgenotype 2.0andAbbott Real-timeHCVGenotype
II)andusingdirectNS5Bsequencing as a gold standard (direct sequencing), in 114 clinical specimenspreviously tested by first-generation
hybridization assay (82 genotype 1and32 with uninterpretable results). Phylogenetic analysis of deep-sequencing reads matched subtype
1 callingbypopulation Sanger sequencing(69%1b,31%1a) in 81 specimensandidentified amixed-subtype infection (1b/3a/1a) in one sample.
Similarly,amongthe 32previously indeterminate specimens, identical genotypeandsubtype results were obtained by directanddeep
sequencing in all but four samples with dual infection. In contrast, both VersantHCVGenotype 2.0andAbbott Real-timeHCVGenotype II
failed subtype 1 calling in 13 (16%) samples eachandwere unable to identify theHCVgenotype and/or subtype inmore than half of the nongenotype
1 samples.Weconcluded that deep sequencing ismore efficient forHCVsubtyping than currently available methodsandallows
qualitative identificationofmixed infectionsandmay bemorehelpfulwith respect to informing treatment strategies withnewDAA-containing
regimens across allHCVsubtypes | en_US |
dc.description.sponsorship | This study has been supported by CDTI (Centro para el Desarrollo Tecnológico
Industrial), Spanish Ministry of Economics and Competitiveness
(MINECO), IDI-20110115; MINECO projects SAF 2009-10403; and
also by the Spanish Ministry of Health, Instituto de Salud Carlos III (FIS)
projects PI10/01505, PI12/01893, and PI13/00456. CIBERehd is funded
by the Instituto de Salud Carlos III, Madrid, Spain. Work at CBMSO was
supported by grant MINECO-BFU2011-23604, FIPSE, and Fundación
Ramón Areces.
X. Forns received unrestricted grant support from Roche and has
acted as advisor for MSD, Gilead, and Abbvie. M. Alvarez-Tejado, J. Gregori,
and J. M. Muñoz work in Roche Diagnostics | en_US |
dc.format.extent | 8 pag. | es_ES |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | American Society for Microbiology | en_US |
dc.relation.ispartof | Journal of Clinical Microbiology | en_US |
dc.rights | © 2015, American Society for Microbiology | en_US |
dc.subject.other | Hepatitis C | en_US |
dc.subject.other | Antivirals | en_US |
dc.subject.other | HCV | en_US |
dc.title | High-resolution hepatitis C virus subtyping using NS5B deep sequencing and phylogeny, an alternative to current methods | en_US |
dc.type | article | en |
dc.subject.eciencia | Medicina | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1128/JCM.02093-14 | es_ES |
dc.identifier.doi | 10.1128/JCM.02093-14 | es_ES |
dc.identifier.publicationfirstpage | 219 | es_ES |
dc.identifier.publicationissue | 1 | es_ES |
dc.identifier.publicationlastpage | 226 | es_ES |
dc.identifier.publicationvolume | 53 | es_ES |
dc.relation.projectID | Gobierno de España. IDI-20110115 | es_ES |
dc.relation.projectID | Gobierno de España. SAF 2009-10403 | es_ES |
dc.relation.projectID | Gobierno de España. BFU2011-23604, | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | en_US |
dc.rights.accessRights | openAccess | en |
dc.facultadUAM | Facultad de Medicina | |
dc.institutoUAM | Centro de Biología Molecular Severo Ochoa (CBMSO) | |