FoxJ2 y FoxJ3 dos factores de transcripcioón de la familia Fork Head: aproximación a su función biológica
Author
Martín de Lara, FernandoAdvisor
Rey Campos, JavierEntity
UAM. Departamento de Biología Molecular; CSIC. Centro de Investigaciones Biológicas (CIB)Date
2005-07-05Subjects
Proteínas - Tesis doctorales; Genética molecular - Tesis doctorales; Biología y Biomedicina / BiologíaNote
Tesis doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular, Fecha de lectura: 05-07-2005Abstract
FoxJ2 is a transcription factor of the Fork head family. It is a positive regulator
of transcription, which is expressed since very early in embryonic development and it is
widely distributed in the adult.
The work described in this thesis aimed the elucidation of the biological function
of FoxJ2. We used transgenic mice to address this issue. We have found that overexpression
of FoxJ2 retarded the progress of pre-implantation development and
produced embryonic lethality at stage E10,5. This results in a small litter size and a very
low number of adult transgenic mice which express the transgén. Furthermore, the
FoxJ2 transgenic suwivors died early showing heart hypertrophy and lung blood edema.
In addition, over-expression of FoxJ2 may cause male infertility, arresting the
spermatogenesis. We also show in this work that FoxJ2 is able to transactivate the
promoters of Conexin-43 and Cadherina-E genes in transfection experiments.
Based on these results, we propose that the lethal effect obsewed at E10,5 in
FoxJ2 transgenic mice may be due to alterations in the transcriptional regulation of
Conexin 43 andlor Cadherina-E. This could also explain the heart hypertrophy and the
male infertility obsewed in FoxJ2 transgenic suwivor mice.
The pattem of expression, in embryonic development and in the adult, of FoxJ3,
the closest evolutionary relative of FoxJ2, is also described in this thesis. The pattem of
expression of Foxj3 partial overlaps that of Foxj2. Similar to this factor, FoxJ3 is
expressed in a broad range of organs and tissues. This, together with the high sequence
consewation between both fork head facton, suggests that they could have partially
redundant functions andfor functional interaction, either positive or negative, may occur
among them.
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