Generation of three-dimensional multiple spheroid model of olfactory ensheathing cells using floating liquid marbles
EntityUAM. Departamento de Biología Molecular
PublisherNature Publishing Group
10.1038/srep15083Scientific Reports 5 (2015): 15083
Funded byJSJ was funded by a grant from the Perry Cross Spinal Research Foundation; NTN was funded from Griffith University through a start-up grant and a grant from the Griffith University Research Infrastructure Program; JAK was funded by an Australian Research Council Discovery Grant DP150104495; JT was funded by an Eskitis Institute scholarship; CO was funded by a Griffith Sciences scholarship; RV was funded by a Griffith University International Postgraduate Research Scholarship
SubjectsOlfactory ensheathing cells (OECs); Three-dimensional; Multiple spheroid model; Liquid marbles; Biología y Biomedicina / Biología
We describe a novel protocol for three-dimensional culturing of olfactory ensheathing cells (OECs), which can be used to understand how OECs interact with other cells in three dimensions. Transplantation of OECs is being trialled for repair of the paralysed spinal cord, with promising but variable results and thus the therapy needs improving. To date, studies of OEC behaviour in a multicellular environment have been hampered by the lack of suitable three-dimensional cell culture models. Here, we exploit the floating liquid marble, a liquid droplet coated with hydrophobic powder and placed on a liquid bath. The presence of the liquid bath increases the humidity and minimises the effect of evaporation. Floating liquid marbles allow the OECs to freely associate and interact to produce OEC spheroids with uniform shapes and sizes. In contrast, a sessile liquid marble on a solid surface suffers from evaporation and the cells aggregate with irregular shapes. We used floating liquid marbles to co-culture OECs with Schwann cells and astrocytes which formed natural structures without the confines of gels or bounding layers. This protocol can be used to determine how OECs and other cell types associate and interact while forming complex cell structures
Google Scholar:Vadivelu, Raja K. - Ooi, Chin H. - Yao, Rebecca Qing - Tello Velasquez, Johana - Pastrana, Erika - Díaz Nido, Javier - Lim, Filip - Ekberg, Jenny A K - Nguyen, Nam Trung - St John, James A.
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Martín Jiménez, Daniel