dc.contributor.author | Zaballos Sánchez, Miguel Ángel | |
dc.contributor.author | Cantero, Walter | |
dc.contributor.author | Azpiazu, Natalia | |
dc.contributor.other | UAM. Departamento de Biología Molecular | es_ES |
dc.date.accessioned | 2016-09-21T13:36:24Z | |
dc.date.available | 2016-09-21T13:36:24Z | |
dc.date.issued | 2015-03-20 | |
dc.identifier.citation | PLoS ONE 10.3 (2015): e0120662 | en_US |
dc.identifier.issn | 1932-6203 (print) | es_ES |
dc.identifier.uri | http://hdl.handle.net/10486/673320 | |
dc.description.abstract | We have previously identified Homothorax (Hth) as an important factor for the correct assembly of the pericentromeric heterochromatin during the first fast syncytial divisions of the Drosophila embryo. Here we have extended our studies to later stages of embryonic development. We were able to show that hth mutants exhibit a drastic overall reduction in the trimethylation of H3 in Lys9, with no reduction of the previous di-methylation. One phenotypic outcome of such a reduction is a genome instability visualized by the many DNA breaks observed in the mutant nuclei. Moreover, loss of Hth leads to the opening of closed heterochromatic regions, including the rDNA genomic region. Our data show that the satellite repeats get transcribed in wild type embryos and that this transcription depends on the presence of Hth, which binds to them as well as to the rDNA region. This work indicates that there is an important role of transcription of non-coding RNAs for constitutive heterochromatin assembly in the Drosophila embryo, and suggests that Hth plays an important role in this process | en_US |
dc.description.sponsorship | Funding from BFU2009-12152 Ministerio de Ciencia e Innovación Fundacion Ramón Areces. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript | en_US |
dc.format.extent | 19 pag. | es_ES |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | Public Library of Science | en_US |
dc.relation.ispartof | PLoS ONE | en_US |
dc.rights | © 2015 Zaballos et al. | en_US |
dc.subject.other | Chromatin immunoprecipitation | en_US |
dc.subject.other | DNA strand breakage | en_US |
dc.subject.other | Drosophila | en_US |
dc.subject.other | Embryo development | en_US |
dc.subject.other | Genomic instability | en_US |
dc.title | The TALE transcription factor Homothorax functions to assemble heterochromatin during Drosophila embryogenesis | en_US |
dc.type | article | en |
dc.subject.eciencia | Biología y Biomedicina / Biología | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1371/journal.pone.0120662 | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0120662 | es_ES |
dc.identifier.publicationfirstpage | 1 | es_ES |
dc.identifier.publicationissue | 3 | es_ES |
dc.identifier.publicationlastpage | 19 | es_ES |
dc.identifier.publicationvolume | 10 | es_ES |
dc.relation.projectID | Gobierno de España. BFU2009-12152 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.rights.cc | Reconocimiento | es_ES |
dc.rights.accessRights | openAccess | en |
dc.facultadUAM | Facultad de Ciencias | |
dc.institutoUAM | Centro de Biología Molecular Severo Ochoa (CBMSO) | |