Particle alignment reliability in single particle electron cryomicroscopy: A general approach
EntityUAM. Departamento de Ingeniería Informática
PublisherNature Publishing Group
10.1038/srep21626Scientific Reports 6 (2016): 21626
Funded byThe authors would like to acknowledge economical support from the Spanish Ministry of Economy and Competitiveness through grants AIC-A-2011-0638 and BIO2013-44647-R, the Comunidad de Madrid through grant CAM (S2010/BMD-2305), and from "Comfuturo" grant funded by the Fundacion General CSIC. C.O.S. Sorzano is recipient of a Ramon y Cajal fellowship. We acknowledge discussions with Sriram Subramanian on validation approaches related to the HIV trimer structure.
ProjectGobierno de España. AIC-A-2011-0638; Gobierno de España. BIO2013-44647-R; Comunidad de Madrid. S2010/BMD-2305/CS INTERACTOMICS
SubjectsCryo-em structure; Hiv-1 envelope glycoproteins; Cryoelectron microscopy; Images; States; Groel; Noise; Informática
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
Electron Microscopy is reaching new capabilities thanks to the combined effect of new technologies and new image processing methods. However, the reconstruction process is still complex, requiring many steps and elaborated optimization procedures. Therefore, the possibility to reach a wrong structure exists, justifying the need of robust statistical tests. In this work, we present a conceptually simple alignment test, which does not require tilt-pair images, to evaluate the alignment consistency between a set of projection images with respect to a given 3D density map. We test the approach on a number of problems in 3DEM, especially the ranking and evaluation of initial 3D volumes and high resolution 3D maps, where we show its usefulness in providing an objective evaluation for maps that have recently been subject to a strong controversy in the field. Additionally, this alignment statistical test can be linked to the early stages of structure solving of new complexes, streamlining the whole process.
Google Scholar:Vargas, Javier - Otón, Joaquín - Marabini Ruiz, Roberto - Carazo, José María - Sorzano, Carlos Oscar
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