Metallacarboranes as tunable redox potential electrochemical indicators for screening of gene mutation
Entity
UAM. Departamento de Química Analítica y Análisis InstrumentalPublisher
Royal Society of ChemistryDate
2016-06-08Citation
10.1039/c6sc01567k
Chemical Science 7.9 (2016): 5786-5797
ISSN
2041-6520 (print); 2041-6539 (online)DOI
10.1039/c6sc01567kFunded by
This work has been supported by the Comunidad Autonoma de Madrid (project No. S2009/PPQ- 363 1642), NANOAVANSENS (project No. S2013/MIT-3029), the Spanish Ministerio de Economia y Competitividad (projects No. CTQ2015- 71955-REDT (ELECTROBIONET), CTQ2014-53334-C2-1 R and CTQ2013-44670 R), and the Generalitat de Catalunya (2014/SGR/149). I. Fuentes is enrolled in the PhD program of the UAB. E. Lorenzo thanks the Fulbright scholarship-Salvador de Madariaga program from Spanish Ministerio de Economia y CompetitividadProject
Comunidad de Madrid. S2009/PPQ-1642/AVANSENS; Comunidad de Madrid. S2013/MIT-3029/NANOAVANSENS; Gobierno de España. CTQ2015- 71955-REDT; Gobierno de España. CTQ2014-53334-C2-1-R; Gobierno de España. CTQ2013-44670-REditor's Version
http://dx.doi.org/10.1039/c6sc01567kSubjects
Chlorine compounds; Polymerase chain reaction; Redox reactions; Metallacarboranes; QuímicaNote
RSC: link from this PDF version to the published article on the Royal Society of Chemistry websiteRights
© 2016 The Royal Society of ChemistryAbstract
The substitution of hydrogen with chlorine in the metallacarborane [3,3-Fe(1,2-closo-C2B9H11)2]- cluster modulates the formal potential of the Fe3+/Fe2+ redox couple, shifting it to a more positive value. Hence, very similar redox probes with a wide range of formal potentials, ranging from negative to positive values, are available. Thus, we have achieved the synthesis and studied the electrochemical behaviour of the sodium salt of [3,3-Fe(8,9,12-Cl3-1,2-closo-C2B9H8)2]- in aqueous media. This strategy allows tuning of the redox potential of the [3,3-Fe(1,2-closo-C2B9H11)2]- framework with a minor change in its shape and dimensions. We also describe the interaction of the prepared [3,3-Fe(8,9,12-Cl3-1,2-closo-C2B9H8)2]- and the pristine [3,3-Fe(1,2-closo-C2B9H11)2]- with DNA. These studies have been carried out not only with DNA in solution but also with DNA immobilized on screen-printed gold electrodes. The results obtained point to a strong interaction between the metallacarboranes and DNA, to a different extent with single stranded DNA (ssDNA) compared to double stranded DNA (dsDNA). This property makes them selective and wide-ranging potential electrochemical indicators of hybridization. The suitability of these new redox indicators for selective DNA biosensor development has been probed by the direct detection of two different mutations associated with cystic fibrosis in PCR amplicons extracted from blood cells
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Google Scholar:García-Mendiola, T.
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Bayon-Pizarro, V.
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Zaulet, A.
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Fuentes, I.
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Pariente Alonso, Félix
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Teixidor, F.
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Viñas, C.
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Lorenzo, E.
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