Synthesis of Oligosaccharides Derived from Lactulose (OsLu) Using Soluble and Immobilized Aspergillus oryzae β-Galactosidase
Entity
UAM. Departamento de Química Física AplicadaPublisher
Frontiers MediaDate
2016-03-07Citation
10.3389/fbioe.2016.00021
Frontiers in Bioengineering and Biotechnology 4 (2016): 21
ISSN
2296-4185 (print)DOI
10.3389/fbioe.2016.00021Funded by
This work has been supported by projects AGL2014-53445-R from Ministerio de Economía y Competitividad; ALIBIRD-CM S-2013/ABI-2728 from Comunidad Autónoma de Madrid and COOPB20099 from CSICProject
Gobierno de España. AGL2014-53445-R; Comunidad de Madrid. S2013/ABI-2728/ALIBIRDEditor's Version
http://dx.doi.org/10.3389/fbioe.2016.00021Subjects
Oligosaccharides; Lactulose; Aspergillus oryzae; Immobilization; Glutaraldehyde–agarose; QuímicaRights
© Frontiers Media S.A. All Rights ReservedAbstract
β-Galactosidase from Aspergillus oryzae offers a high yield for the synthesis of oligosaccharides derived from lactulose (OsLu) by transgalactosylation. Oligosaccharides with degree of polymerization (DP) ≥ 3 have shown to possess higher in vitro bifidogenic effect than di- and tetrasaccharides. Thus, in this work, an optimization of reaction conditions affecting the specific selectivity of A. oryzae β-galactosidase for synthesis of OsLu has been carried out to enhance OsLu with DP ≥ 3 production. Assays with β-galactosidase immobilized onto a glutaraldehyde–agarose support were also carried out with the aim of making the process cost-effective and industrially viable. Optimal conditions with both soluble and immobilized enzyme for the synthesis of OsLu with DP ≥ 3 were 50 °C, pH 6.5, 450 g/L of lactulose, and 8 U/mL of enzyme, reaching yields of ca. 50% (w/v) of total OsLu and ca. 20% (w/v) of OsLu with DP 3, being 6′-galactosyl-lactulose the major one, after a short reaction time. Selective formation of disaccharides, however, was favored at 60 °C, pH 4.5, 450 g/L of lactulose and 8 U/mL of enzyme. Immobilization increased the enzymatic stability to temperature changes and allowed to reuse the enzyme. We can conclude that the use, under determined optimal conditions, of the A. oryzae β-galactosidase immobilized on a support of glutaraldehyde–agarose constitutes an efficient and cost-effective alternative to the use of soluble β-galactosidases for the synthesis of prebiotic OsLu mixtures
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Google Scholar:Cardelle-Cobas, A.
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Olano, A.
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Irazoqui, G.
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Giacomini, C.
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Batista-Viera, F.
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Corzo, N.
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Corzo-Martínez, M.
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