Stallion sperm quality after combined ejaculate fractionation and colloidal centrifugation
EntityUAM. Departamento de Biología
PublisherInstituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria; Ministerio de Agricultura Pesca y Alimentacion
10.5424/sjar/2015134-8108Spanish Journal of Agricultural Research 13.4 (2015): e04SC02
ISSN1695-971X (print); 2171-9292 (online)
Funded byThis research was supported by the Spanish Ministry of Economy and Competitiveness, MINECO (BFU-2013-44290-R)
ProjectGobierno de España. BFU-2013-44290-R
SubjectsEjaculate fractionation; Colloidal centrifugation; Sperm DNA fragmentation; DNA longevity; Equus caballus; SCD; Biología y Biomedicina / Biología
Rights© 2015 INIA
Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial 4.0 Internacional.
This study investigated the possible additive benefit of ejaculate fractionation and colloidal centrifugation on stallion sperm quality. Using an open-end artificial vagina, the sperm-rich fraction (FRAC-1) was separated from the rest of the ejaculate (FRAC-2) and a third sperm sample representing the combined ejaculate was reconstituted post-ejaculation (RAW). Each semen sample was processed for colloidal centrifugation. The percentage of abnormal spermatozoa was 17.8 ± 7.0% in RAW and 14.6 ± 9.5% in FRAC-1 but decreased to 11.4 ± 4.7% and 9.6 ± 6.9% respectively, after colloidal centrifugation. A sperm DNA fragmentation index of 10.9 ± 5.1% was observed in RAW and 7.5 ± 2.4% in FRAC-1 semen collected with the AV but this decreased to 7.8 ± 2.8% and 5.2 ± 2.3% after colloidal centrifugation. The rate of increase in sperm DNA fragmentation during the first 6 h of incubation at 37 ºC was 1.8 ± 0.9% per hour in RAW semen and 2.0 ± 2.0% per hour in FRAC-1 but this significantly decreased to 1.3 ± 1.4% and 0.9 ± 0.8% respectively after colloidal centrifugation. While stallion seminal characteristics can be improved using colloidal centrifugation, further enhancement is possible if the ejaculate is initially fractionated
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