Stallion sperm quality after combined ejaculate fractionation and colloidal centrifugation
Entity
UAM. Departamento de BiologíaPublisher
Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria; Ministerio de Agricultura Pesca y AlimentacionDate
2015-11-10Citation
10.5424/sjar/2015134-8108
Spanish Journal of Agricultural Research 13.4 (2015): e04SC02
ISSN
1695-971X (print); 2171-9292 (online)DOI
10.5424/sjar/2015134-8108Funded by
This research was supported by the Spanish Ministry of Economy and Competitiveness, MINECO (BFU-2013-44290-R)Project
Gobierno de España. BFU-2013-44290-REditor's Version
http://dx.doi.org/10.5424/sjar/2015134-8108Subjects
Ejaculate fractionation; Colloidal centrifugation; Sperm DNA fragmentation; DNA longevity; Equus caballus; SCD; Biología y Biomedicina / BiologíaRights
© 2015 INIAAbstract
This study investigated the possible additive benefit of ejaculate fractionation and colloidal centrifugation on stallion sperm quality. Using an open-end artificial vagina, the sperm-rich fraction (FRAC-1) was separated from the rest of the ejaculate (FRAC-2) and a third sperm sample representing the combined ejaculate was reconstituted post-ejaculation (RAW). Each semen sample was processed for colloidal centrifugation. The percentage of abnormal spermatozoa was 17.8 ± 7.0% in RAW and 14.6 ± 9.5% in FRAC-1 but decreased to 11.4 ± 4.7% and 9.6 ± 6.9% respectively, after colloidal centrifugation. A sperm DNA fragmentation index of 10.9 ± 5.1% was observed in RAW and 7.5 ± 2.4% in FRAC-1 semen collected with the AV but this decreased to 7.8 ± 2.8% and 5.2 ± 2.3% after colloidal centrifugation. The rate of increase in sperm DNA fragmentation during the first 6 h of incubation at 37 ºC was 1.8 ± 0.9% per hour in RAW semen and 2.0 ± 2.0% per hour in FRAC-1 but this significantly decreased to 1.3 ± 1.4% and 0.9 ± 0.8% respectively after colloidal centrifugation. While stallion seminal characteristics can be improved using colloidal centrifugation, further enhancement is possible if the ejaculate is initially fractionated
Files in this item
Google Scholar:Crespo, F.
-
Gosalbez Berenguer, José Jaime
-
Johnston, S.D.
-
De la Torre, J.
This item appears in the following Collection(s)
Related items
Showing items related by title, author, creator and subject.