Molecular genetics of naringenin biosynthesis, a typical plant secondary metabolite produced by Streptomyces clavuligerus
Entity
UAM. Departamento de Química OrgánicaPublisher
BioMed CentralDate
2015-11-09Citation
10.1186/s12934-015-0373-7
Microbial Cell Factories 14.1 (2015): 178
ISSN
1475-2859DOI
10.1186/s12934-015-0373-7Funded by
This work was supported by Grant BIO2012-34723 from the Spanish Ministry of Economy and Competitivity. R. Álvarez-Álvarez received a FPU fellowship from the Spanish Ministry of Education, Culture and SportsProject
Gobierno de España. BIO2012-34723Editor's Version
http://dx.doi.org/10.1186/s12934-015-0373-7Subjects
Histidine/phenylalanine ammonium lyase; Naringenin; Secondary metabolism; Streptomyces clavuligerus; QuímicaRights
© 2015 Álvarez-Álvarez et alAbstract
Background: Some types of flavonoid intermediates seemed to be restricted to plants. Naringenin is a typical plant metabolite, that has never been reported to be produced in prokariotes. Naringenin is formed by the action of a chalcone synthase using as starter 4-coumaroyl-CoA, which in dicotyledonous plants derives from phenylalanine by the action of a phenylalanine ammonia lyase. Results: A compound produced by Streptomyces clavuligerus has been identified by LC-MS and NMR as naringenin and coelutes in HPLC with a naringenin standard. Genome mining of S. clavuligerus revealed the presence of a gene for a chalcone synthase (ncs), side by side to a gene encoding a P450 cytochrome (ncyP) and separated from a gene encoding a Pal/Tal ammonia lyase (tal). Deletion of any of these genes results in naringenin non producer mutants. Complementation with the deleted gene restores naringenin production in the transformants. Furthermore, naringenin production increases in cultures supplemented with phenylalanine or tyrosine. Conclusion: This is the first time that naringenin is reported to be produced naturally in a prokariote. Interestingly three non-clustered genes are involved in naringenin production, which is unusual for secondary metabolites. A tentative pathway for naringenin biosynthesis has been proposed
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Google Scholar:Álvarez‑Álvarez, R.
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Botas, A.
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Albillos, S.
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Rumbero Sánchez, Ángel
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Martín, J.
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Liras, P.
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