Gene and whole genome analyses reveal that the mycobacterial strain JS623 is not a member of the species Mycobacterium smegmatis
Entity
UAM. Departamento de Medicina Preventiva y Salud Pública y MicrobiologíaPublisher
John Wiley & Sons Ltd and SocietyDate
2016Citation
10.1111/1751-7915.12336
Microbial Biotechnology 9.2 (2016): 269-274
ISSN
1751-7915DOI
10.1111/1751-7915.12336Funded by
This work has been partially supported by funds from the International Cooperation UAM-Banco Santander and Latin America (CEAL-UAM) and the Spanish Ministry of Health (AES, PI13-01218) (MJG) as well as BFU2014-52070-C2-1 1 from the Spanish Government (SG)Project
Gobierno de España. BFU2014-52070-C2-1Editor's Version
http://dx.doi.org/10.1111/1751-7915.12336Subjects
Gene; Genome analyses; Mycobacterium smegmatis; Molecular taxonomic; MedicinaRights
© 2016 The AuthorAbstract
Unexpected differences were found between the
genome of strain JS623, used in bioremediation
studies, and the genome of strain mc2155, a model
organism for investigating basic biology of
mycobacteria. Both strains are currently assigned
in the databases to the species Mycobacterium
smegmatis and, consequently, the environmental
isolate JS623 is increasingly included as a representative
of that species in comparative genomebased
approaches aiming at identifying distinctive
traits of the different members of the genus Mycobacterium.
We applied traditional molecular taxonomic
procedures – inference of single and concatenated
gene trees – to re-evaluate the membership of both
strains to the same species, adopting the latest
accepted cut-off values for species delimitation.
Additionally, modern whole genome-based in silico
methods where performed in a comprehensive
molecular phylogenetic analysis of JS623 and other
members of the genus Mycobacterium. These analyses
showed that all relevant genome parameters of
JS623 clearly separate this strain from M. smegmatis.
The strain JS623 should be corrected as Mycobacterium
sp. not only in the literature but, even more
importantly, in the database entries, as inclusion of
the genome wrongly attributed to the M. smegmatis
species in comparative studies will result in misleading
conclusions
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Google Scholar:García García, María Jesús
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Gola, Susanne
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