Mañana, JUEVES, 24 DE ABRIL, el sistema se apagará debido a tareas habituales de mantenimiento a partir de las 9 de la mañana. Lamentamos las molestias.
Ghrelin regulates glucose and glutamate transporters in hypothalamic astrocytes
Entity
UAM. Departamento de Fisiología; UAM. Departamento de Medicina; UAM. Departamento de Pediatría; Instituto de Investigación del Hospital de La Princesa (IP)Publisher
Nature Publishing GroupDate
2016-03-30Citation
10.1038/srep23673
Scientific Reports 6 (2016): 23673
ISSN
2045-2322DOI
10.1038/srep23673Funded by
This work was funded by grants from Fondo de Investigación Sanitaria (PI100747; PI1302195), Ministerio de Ciencia e Innovación (BFU2011–27492; BFU2014-51836-C2-2-R) and Fondos FEDER, Centro de Investigación Biomédica en Red Fisiopatología de Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, and Fundación de Endocrinología y Nutrición. SD was funded by the Swedish Research Council (Vetenskapsrådet grant 2012– 1758), Läkarutbildningsavtalet Göteborg grant at Sahlgrenska Hospital (ALFGBG-138741, The European Union Seventh Framework Programme under Grant Agreement 607310, Nudge-it)Project
Gobierno de España. BFU2011–27492; Gobierno de España. BFU2014-51836-C2-2-R; info:eu-repo/grantAgreement/EC/FP7/607310Editor's Version
http://dx.doi.org/10.1038/srep23673Subjects
Insulin; Glucose; Ghrelin; 1A (GHSR-1A); GLUT2; Signals/nutrients; MedicinaAbstract
Hypothalamic astrocytes can respond to metabolic signals, such as leptin and insulin, to modulate adjacent neuronal circuits and systemic metabolism. Ghrelin regulates appetite, adiposity and glucose metabolism, but little is known regarding the response of astrocytes to this orexigenic hormone. We have used both in vivo and in vitro approaches to demonstrate that acylated ghrelin (acyl-ghrelin) rapidly stimulates glutamate transporter expression and glutamate uptake by astrocytes. Moreover, acyl-ghrelin rapidly reduces glucose transporter (GLUT) 2 levels and glucose uptake by these glial cells. Glutamine synthetase and lactate dehydrogenase decrease, while glycogen phosphorylase and lactate transporters increase in response to acyl-ghrelin, suggesting a change in glutamate and glucose metabolism, as well as glycogen storage by astrocytes. These effects are partially mediated through ghrelin receptor 1A (GHSR-1A) as astrocytes do not respond equally to desacyl-ghrelin, an isoform that does not activate GHSR-1A. Moreover, primary astrocyte cultures from GHSR-1A knock-out mice do not change glutamate transporter or GLUT2 levels in response to acyl-ghrelin. Our results indicate that acyl-ghrelin may mediate part of its metabolic actions through modulation of hypothalamic astrocytes and that this effect could involve astrocyte mediated changes in local glucose and glutamate metabolism that alter the signals/nutrients reaching neighboring neurons.
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Google Scholar:Fuente-Martín, Esther
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García-Cáceres, Cristina
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Argente-Arizón, Pilar
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Díaz, Francisca
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Granado García, Miriam
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Freire-Regatillo, Alejandra
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Castro-González, David
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Ceballos, María L.
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Frago Fernández, Laura María
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Dickson, Suzanne L.
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Argente Oliver, Jesús
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Chowen, Julie Ann
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