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Characterization of interaction of magnetic nanoparticles with breast cancer cells

Author
Calero, Macarena; Chiappi, Michele; Lazaro-Carrillo, Ana; Rodríguez, María José; Chichón, Francisco Javier; Crosbie-Staunton, Kieran; Prina-Mello, Adriele; Volkov, Yuri; Villanueva, Angeles; Carrascosa, José L.
Entity
UAM. Departamento de Biología
Publisher
BioMed Central Ltd.
Date
2015-02-26
Citation
10.1186/s12951-015-0073-9
Journal of Nanobiotechnology 13.1 (2015): 16
 
 
 
ISSN
1477-3155 (print)
DOI
10.1186/s12951-015-0073-9
Funded by
The research leading to these results have received partial funding from the European Seventh Framework Programme (FP7/2007-2013) under the project MULTIFUN grant agreement no. 262943, and the project Nanofrontmag-CM (S2013/MIT-2850) from the Comunidad de Madrid. Additional grants were obtained from BFU 2011–29038 and CTQ2013-48767-C3-3-R from the Ministerio de Economia y Competitividad and S2009/Mat 1507 from the Comunidad de Madrid (to JLC), from EU FP7 project NAMDIATREAM (ref 246479) and from “la Caixa” / CNB International PhD Programme Fellowships
Project
info:eu-repo/grantAgreement/EC/FP7/262943; Comunidad de Madrid. S2013/MIT-2850/NANOFRONTMAG; Gobierno de España. BFU 2011–29038; Gobierno de España. CTQ2013-48767-C3-3-R; Comunidad de Madrid. S2009/MAT-1507/NOBIMAT; info:eu-repo/grantAgreement/EC/FP7/246479
Editor's Version
http://dx.doi.org/10.1186/s12951-015-0073-9
Subjects
Cellular uptake; Cytotoxicity; Endocytosis; Intracellular trafficking; MCF-7 cells; Superparamagnetic iron oxide nanoparticles; Transmission electron microscopy; Biología y Biomedicina / Biología
URI
http://hdl.handle.net/10486/678774
Rights
© 2015 Calero et al

Licencia Creative Commons
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.

Abstract

Background: Different superparamagnetic iron oxide nanoparticles have been tested for their potential use in cancer treatment, as they enter into cells with high effectiveness, do not induce cytotoxicity, and are retained for relatively long periods of time inside the cells. We have analyzed the interaction, internalization and biocompatibility of dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles with an average diameter of 15 nm and negative surface charge in MCF-7 breast cancer cells. Results: Cells were incubated with dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles for different time intervals, ranging from 0.5 to 72 h. These nanoparticles showed efficient internalization and relatively slow clearance. Time-dependent uptake studies demonstrated the maximum accumulation of dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles after 24 h of incubation, and afterwards they were slowly removed from cells. Superparamagnetic iron oxide nanoparticles were internalized by energy dependent endocytosis and localized in endosomes. Transmission electron microscopy studies showed macropinocytosis uptake and clathrin-mediated internalization depending on the nanoparticles aggregate size. MCF-7 cells accumulated these nanoparticles without any significant effect on cell morphology, cytoskeleton organization, cell cycle distribution, reactive oxygen species generation and cell viability, showing a similar behavior to untreated control cells. Conclusions: All these findings indicate that dimercaptosuccinic acid-coated superparamagnetic iron oxide nanoparticles have excellent properties in terms of efficiency and biocompatibility for application to target breast cancer cells
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Google™ Scholar:Calero, Macarena - Chiappi, Michele - Lazaro-Carrillo, Ana - Rodríguez, María José - Chichón, Francisco Javier - Crosbie-Staunton, Kieran - Prina-Mello, Adriele - Volkov, Yuri - Villanueva, Angeles - Carrascosa, José L.

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