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dc.contributor.authorCuadros, Juan
dc.contributor.authorPérez-Tanoira, Ramón
dc.contributor.authorPrieto Pérez, Laura 
dc.contributor.authorMartin- Martin, Inés
dc.contributor.authorBerzosa, Pedro
dc.contributor.authorGonzález, Vicenta
dc.contributor.authorTisiano, Gebre
dc.contributor.authorBalcha, Seble
dc.contributor.authorRamos, José Manuel
dc.contributor.authorGórgolas Hernández-Mora, Miguel 
dc.contributor.otherUAM. Departamento de Medicinaes_ES
dc.contributor.otherInstituto de Investigación Sanitaria Fundación Jiménez Díaz (IIS-FJD)es_ES
dc.date.accessioned2017-07-14T15:59:07Z
dc.date.available2017-07-14T15:59:07Z
dc.date.issued2015-11
dc.identifier.citationPLoS ONE 10.11 (2015): e0142842en_US
dc.identifier.issn1932-6203es_ES
dc.identifier.urihttp://hdl.handle.net/10486/679043
dc.description.abstractBackground In up to one third of the hospitals in some rural areas of Africa, laboratory services in malaria diagnosis are limited to microscopy by thin film, as no capability to perform thick film exists (gold standard in terms of sensitivity for malaria diagnosis). A new rapid molecular malaria diagnostic test called Loop-mediated isothermal DNA amplification (LAMP) has been recently validated in clinical trials showing exceptional sensitivity and specificity features. It could be a reliable diagnostic tool to be implemented without special equipment or training. Objective The objective of this proof of concept study was to confirm the feasibility of using LAMP technique for diagnosis of malaria in a rural Ethiopian hospital with limited resources. Methodology/Principal Findings This study was carried out in Gambo General Hospital, West Arsi Province (Ethiopia), from November 1st to December 31st 2013. A total of 162 patients with a non-focal febrile syndrome were investigated. The diagnostic capability (sensitivity, specificity, positive predictive and negative predictive values) of rapid malaria tests and microscopy by thin film was evaluated in comparison with LAMP. Eleven (6.79%) out of the 162 patients with fever and suspected malaria, tested positive for LAMP, 3 (1.85%) for rapid malaria tests and none of the eleven cases was detected by thin film microscopy. Conclusions/Significance LAMP can be performed in basic rural laboratories without the need for specialized infrastructure and it may set a reliable tool for malaria control to detect a low level parasitemiaen_US
dc.description.sponsorshipThe study was funded by the Master of Tropical diseases of Department of Medicine, Universidad Autónoma de Madrid and Rural Gambo Hospital, Ethiopia. The RDTs were provided by the government of Ethiopiaen_US
dc.format.extent8 pag.es_ES
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofPLoS ONEen_US
dc.rights© 2015 Cuadros et al.es_ES
dc.subject.otherHospitalsen_US
dc.subject.otherMalariaen_US
dc.subject.otherRural areas Africaen_US
dc.subject.otherDiagnosisen_US
dc.subject.otherMicroscopyen_US
dc.subject.otherEthiopiaen_US
dc.titleField evaluation of malaria microscopy, rapid malaria tests and loop-mediated isothermal amplification in a rural hospital in south western Ethiopiaen_US
dc.typearticleen
dc.subject.ecienciaMedicinaes_ES
dc.relation.publisherversionhttps://doi.org/10.1371/ journal.pone.0142842es_ES
dc.identifier.doi10.1371/journal.pone.0142842es_ES
dc.identifier.publicationfirstpagee0142842-1es_ES
dc.identifier.publicationissue11es_ES
dc.identifier.publicationlastpagee0142842-8es_ES
dc.identifier.publicationvolume10es_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.rights.ccReconocimientoes_ES
dc.rights.accessRightsopenAccessen
dc.authorUAMGórgolas Hernández-Mora, Miguel (261167)
dc.facultadUAMFacultad de Medicina
dc.institutoUAMInstituto de Investigación Sanitaria Fundación Jiménez Díaz (ISS-FJD)


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