Polarization-sensitive optical projection tomography for muscle fiber imaging
EntityUAM. Departamento de Biología Molecular
PublisherSpringer Nature; Nature Publishing Group
10.1038/srep19241Scientific Reports 2016.6 (2016): 19241
Funded byThis work is supported by the National Basic Research Program of China (973 Program) under Grant 2011CB707700, the National Natural Science Foundation of China under Grant No. 81227901, 61231004, 81501616, 81301346, 81527805 the Chinese Academy of Sciences Fellowship for Young Foreign Scientists under Grant No. 2010Y2GA03, 2013Y1GA0004, the Chinese Academy of Sciences Visiting Professorship for Senior International Scientists under Grant No. 2012T1G0036, 2013T1G0013,the Instrument Developing Project of the Chinese Academy of Sciences under Grant No. YZ201502, YZ201457 and the Strategic Priority Research Program (B) of Chinese Academy of Sciences (XDB02060010). A. Arranz acknowledges support from the Marie Curie Intra-European Fellowship program IEF-2010-275137. J.R. acknowledges support from EC FP7 IMI project PREDICT-TB, the EC FP7 CIG grant HIGH-THROUGHPUT TOMO, and the Spanish MINECO project grant FIS2013-41802-R MESO-IMAGING
Projectinfo:eu-repo/grantAgreement/EC/FP7/275137; Gobierno de España. FIS2013-41802-R; info:eu-repo/grantAgreement/EC/FP7/115337
SubjectsOptical tomography; Sensitivity and specificity; Animals; Three dimensional imaging; Biología y Biomedicina / Biología
Rights© The Author(s) 2016
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
Optical projection tomography (OPT) is a tool used for three-dimensional imaging of millimeter-scale biological samples, with the advantage of exhibiting isotropic resolution typically in the micron range. OPT can be divided into two types: transmission OPT (tOPT) and emission OPT (eOPT). Compared with eOPT, tOPT discriminates different tissues based on their absorption coefficient, either intrinsic or after specific staining. However, it fails to distinguish muscle fibers whose absorption coefficients are similar to surrounding tissues. To circumvent this problem, in this article we demonstrate a polarization sensitive OPT system which improves the detection and 3D imaging of muscle fibers by using polarized light. We also developed image acquisition and processing protocols that, together with the system, enable the clear visualization of muscles. Experimental results show that the muscle fibers of diaphragm and stomach, difficult to be distinguished in regular tOPT, were clearly displayed in our system, proving its potential use. Moreover, polarization sensitive OPT was fused with tOPT to investigate the stomach tissue comprehensively. Future applications of polarization sensitive OPT could be imaging other fiberlike structures such as myocardium or other tissues presenting high optical anisotropy
Google Scholar:Fang, Mengjie - Dong, Di - Zeng, Chaoting - Liang, Xiao - Yang, Xin - Arranz, Alicia - Ripoll, Jorge - Hui, Hui - Tian, Jie
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