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Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models

Author
Solana, José Carlos; Ramírez, Laura; Corvo, Laura; Oliveira, Camila Indiani de; Barral-Netto, Manoel; Requena Rolania, José Maríauntranslated; Iborra, Salvador; Soto Álvarez, Manueluntranslated
Entity
UAM. Departamento de Biología Molecular; Centro de Biología Molecular Severo Ochoa (CBM)
Publisher
Public Library of Science
Date
2017-05-30
Citation
10.1371/journal.pntd.0005644
PLos Neglected Tropical Diseases 11.5 (2017): e0005644.
 
 
 
ISSN
1935-2727 (print); 1935-2735 (online)
DOI
10.1371/journal.pntd.0005644
Funded by
The study was supported in Spain by grants from Ministerio de Ciencia e Innovación FISPI14/00366 (FEDER FUNDING) from the Heath Institute Carlos III (ISCIII). JCS was supported by the European Community Seventh Framework Programme under grant agreement No. 603181 (Project MuLeVaClin), and LC was supported by a grant from Fondo de Investigaciones Sanitarias (ISCIII-RETICRD16/0027/0008-FEDER). A Brazilian grant from CNPq within the Ciencia sem Fronteiras-PVE program (Ref: 300174/2014-4) is also acknowledged. Finally, we also acknowledge the funding from the EU 7th Framework Programme under grant agreement No. 603181 (MuLeVaClin). A CBMSO institutional grant from Fundación Ramón Areces is also acknowledged
Project
Gobierno de España. FISPI14/00366; info:eu-repo/grantAgreement/EC/FP7/603181; Gobierno de España. ISCIII-RETICRD16/0027/0008-FEDER
Editor's Version
https://doi.org/10.1371/journal.pntd.0005644
Subjects
Leishmania; HSP70-II genes; LiΔHSP70-II; C57BL/6; Infantum; Infection; Biología y Biomedicina / Biología
URI
http://hdl.handle.net/10486/680146
Rights
© 2017 Solana et al.

Licencia Creative Commons
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.

Abstract

Background The immunization with genetically attenuated Leishmania cell lines has been associated to the induction of memory and effector T cell responses against Leishmania able to control subsequent challenges. A Leishmania infantum null mutant for the HSP70-II genes has been described, possessing a non-virulent phenotype. Methodology/Principal findings The L. infantum attenuated parasites (LiΔHSP70-II) were inoculated in BALB/c (intravenously and subcutaneously) and C57BL/6 (subcutaneously) mice. An asymptomatic infection was generated and parasites diminished progressively to become undetectable in most of the analyzed organs. However, inoculation resulted in the long-term induction of parasite specific IFN-γ responses able to control the disease caused by a challenge of L. major infective promastigotes. BALB/c susceptible mice showed very low lesion development and a drastic decrease in parasite burdens in the lymph nodes draining the site of infection and internal organs. C57BL/6 mice did not show clinical manifestation of disease, correlated to the rapid migration of Leishmania specific IFN-γ producing T cells to the site of infection. Conclusion/Significance Inoculation of the LiΔHSP70-II attenuated line activates mammalian immune system for inducing moderate pro-inflammatory responses. These responses are able to confer longterm protection in mice against the infection of L. major virulent parasites
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Google™ Scholar:Solana, José Carlos - Ramírez, Laura - Corvo, Laura - Oliveira, Camila Indiani de - Barral-Netto, Manoel - Requena Rolania, José María - Iborra, Salvador - Soto Álvarez, Manuel

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  • Producción científica en acceso abierto de la UAM [16646]

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Universidad Autónoma de Madrid. Biblioteca
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