Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models
Entity
UAM. Departamento de Biología Molecular; Centro de Biología Molecular Severo Ochoa (CBM)Publisher
Public Library of ScienceDate
2017-05-30Citation
10.1371/journal.pntd.0005644
PLos Neglected Tropical Diseases 11.5 (2017): e0005644.
ISSN
1935-2727 (print); 1935-2735 (online)DOI
10.1371/journal.pntd.0005644Funded by
The study was supported in Spain by grants from Ministerio de Ciencia e Innovación FISPI14/00366 (FEDER FUNDING) from the Heath Institute Carlos III (ISCIII). JCS was supported by the European Community Seventh Framework Programme under grant agreement No. 603181 (Project MuLeVaClin), and LC was supported by a grant from Fondo de Investigaciones Sanitarias (ISCIII-RETICRD16/0027/0008-FEDER). A Brazilian grant from CNPq within the Ciencia sem Fronteiras-PVE program (Ref: 300174/2014-4) is also acknowledged. Finally, we also acknowledge the funding from the EU 7th Framework Programme under grant agreement No. 603181 (MuLeVaClin). A CBMSO institutional grant from Fundación Ramón Areces is also acknowledgedProject
Gobierno de España. FISPI14/00366; info:eu-repo/grantAgreement/EC/FP7/603181; Gobierno de España. ISCIII-RETICRD16/0027/0008-FEDEREditor's Version
https://doi.org/10.1371/journal.pntd.0005644Subjects
Leishmania; HSP70-II genes; LiΔHSP70-II; C57BL/6; Infantum; Infection; Biología y Biomedicina / BiologíaRights
© 2017 Solana et al.Abstract
Background
The immunization with genetically attenuated Leishmania cell lines has been associated to
the induction of memory and effector T cell responses against Leishmania able to control
subsequent challenges. A Leishmania infantum null mutant for the HSP70-II genes has
been described, possessing a non-virulent phenotype.
Methodology/Principal findings
The L. infantum attenuated parasites (LiΔHSP70-II) were inoculated in BALB/c (intravenously
and subcutaneously) and C57BL/6 (subcutaneously) mice. An asymptomatic infection
was generated and parasites diminished progressively to become undetectable in most
of the analyzed organs. However, inoculation resulted in the long-term induction of parasite
specific IFN-γ responses able to control the disease caused by a challenge of L. major infective
promastigotes. BALB/c susceptible mice showed very low lesion development and a
drastic decrease in parasite burdens in the lymph nodes draining the site of infection and
internal organs. C57BL/6 mice did not show clinical manifestation of disease, correlated to
the rapid migration of Leishmania specific IFN-γ producing T cells to the site of infection.
Conclusion/Significance
Inoculation of the LiΔHSP70-II attenuated line activates mammalian immune system for
inducing moderate pro-inflammatory responses. These responses are able to confer longterm
protection in mice against the infection of L. major virulent parasites
Files in this item
Google Scholar:Solana, José Carlos
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Ramírez, Laura
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Corvo, Laura
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Oliveira, Camila Indiani de
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Barral-Netto, Manoel
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Requena Rolania, José María
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Iborra, Salvador
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Soto Álvarez, Manuel
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