Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models
EntityUAM. Departamento de Biología Molecular; Centro de Biología Molecular Severo Ochoa (CBM)
PublisherPublic Library of Science
10.1371/journal.pntd.0005644PLos Neglected Tropical Diseases 11.5 (2017): e0005644.
ISSN1935-2727 (print); 1935-2735 (online)
Funded byThe study was supported in Spain by grants from Ministerio de Ciencia e Innovación FISPI14/00366 (FEDER FUNDING) from the Heath Institute Carlos III (ISCIII). JCS was supported by the European Community Seventh Framework Programme under grant agreement No. 603181 (Project MuLeVaClin), and LC was supported by a grant from Fondo de Investigaciones Sanitarias (ISCIII-RETICRD16/0027/0008-FEDER). A Brazilian grant from CNPq within the Ciencia sem Fronteiras-PVE program (Ref: 300174/2014-4) is also acknowledged. Finally, we also acknowledge the funding from the EU 7th Framework Programme under grant agreement No. 603181 (MuLeVaClin). A CBMSO institutional grant from Fundación Ramón Areces is also acknowledged
ProjectGobierno de España. FISPI14/00366; info:eu-repo/grantAgreement/EC/FP7/603181; Gobierno de España. ISCIII-RETICRD16/0027/0008-FEDER
SubjectsLeishmania; HSP70-II genes; LiΔHSP70-II; C57BL/6; Infantum; Infection; Biología y Biomedicina / Biología
Rights© 2017 Solana et al.
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
Background The immunization with genetically attenuated Leishmania cell lines has been associated to the induction of memory and effector T cell responses against Leishmania able to control subsequent challenges. A Leishmania infantum null mutant for the HSP70-II genes has been described, possessing a non-virulent phenotype. Methodology/Principal findings The L. infantum attenuated parasites (LiΔHSP70-II) were inoculated in BALB/c (intravenously and subcutaneously) and C57BL/6 (subcutaneously) mice. An asymptomatic infection was generated and parasites diminished progressively to become undetectable in most of the analyzed organs. However, inoculation resulted in the long-term induction of parasite specific IFN-γ responses able to control the disease caused by a challenge of L. major infective promastigotes. BALB/c susceptible mice showed very low lesion development and a drastic decrease in parasite burdens in the lymph nodes draining the site of infection and internal organs. C57BL/6 mice did not show clinical manifestation of disease, correlated to the rapid migration of Leishmania specific IFN-γ producing T cells to the site of infection. Conclusion/Significance Inoculation of the LiΔHSP70-II attenuated line activates mammalian immune system for inducing moderate pro-inflammatory responses. These responses are able to confer longterm protection in mice against the infection of L. major virulent parasites
Google Scholar:Solana, José Carlos - Ramírez, Laura - Corvo, Laura - Oliveira, Camila Indiani de - Barral-Netto, Manoel - Requena Rolania, José María - Iborra, Salvador - Soto Álvarez, Manuel
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