Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models

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Show simple item record Solana, José Carlos Ramírez, Laura Corvo, Laura Oliveira, Camila Indiani de Barral-Netto, Manoel Requena, José M. Iborra, Salvador Soto Álvarez, Manuel
dc.contributor.other UAM. Departamento de Biología Molecular es_ES
dc.contributor.other Centro de Biología Molecular Severo Ochoa (CBMSO) es_ES 2017-11-06T17:18:07Z 2017-11-06T17:18:07Z 2017-05-30
dc.identifier.citation PLos Neglected Tropical Diseases 11.5 (2017): e0005644. en_US
dc.identifier.issn 1935-2727 (print) es_ES
dc.identifier.issn 1935-2735 (online) es_ES
dc.description.abstract Background The immunization with genetically attenuated Leishmania cell lines has been associated to the induction of memory and effector T cell responses against Leishmania able to control subsequent challenges. A Leishmania infantum null mutant for the HSP70-II genes has been described, possessing a non-virulent phenotype. Methodology/Principal findings The L. infantum attenuated parasites (LiΔHSP70-II) were inoculated in BALB/c (intravenously and subcutaneously) and C57BL/6 (subcutaneously) mice. An asymptomatic infection was generated and parasites diminished progressively to become undetectable in most of the analyzed organs. However, inoculation resulted in the long-term induction of parasite specific IFN-γ responses able to control the disease caused by a challenge of L. major infective promastigotes. BALB/c susceptible mice showed very low lesion development and a drastic decrease in parasite burdens in the lymph nodes draining the site of infection and internal organs. C57BL/6 mice did not show clinical manifestation of disease, correlated to the rapid migration of Leishmania specific IFN-γ producing T cells to the site of infection. Conclusion/Significance Inoculation of the LiΔHSP70-II attenuated line activates mammalian immune system for inducing moderate pro-inflammatory responses. These responses are able to confer longterm protection in mice against the infection of L. major virulent parasites en_US
dc.description.sponsorship The study was supported in Spain by grants from Ministerio de Ciencia e Innovación FISPI14/00366 (FEDER FUNDING) from the Heath Institute Carlos III (ISCIII). JCS was supported by the European Community Seventh Framework Programme under grant agreement No. 603181 (Project MuLeVaClin), and LC was supported by a grant from Fondo de Investigaciones Sanitarias (ISCIII-RETICRD16/0027/0008-FEDER). A Brazilian grant from CNPq within the Ciencia sem Fronteiras-PVE program (Ref: 300174/2014-4) is also acknowledged. Finally, we also acknowledge the funding from the EU 7th Framework Programme under grant agreement No. 603181 (MuLeVaClin). A CBMSO institutional grant from Fundación Ramón Areces is also acknowledged en_US
dc.format.extent 26 pag. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Public Library of Science en_US
dc.relation.ispartof PLos Neglected Tropical Diseases en_US
dc.rights © 2017 Solana et al. es_ES
dc.subject.other Leishmania en_US
dc.subject.other HSP70-II genes en_US
dc.subject.other LiΔHSP70-II en_US
dc.subject.other C57BL/6 en_US
dc.subject.other Infantum en_US
dc.subject.other Infection en_US
dc.title Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models en_US
dc.type article en
dc.subject.eciencia Biología y Biomedicina / Biología es_ES
dc.relation.publisherversion es_ES
dc.identifier.doi 10.1371/journal.pntd.0005644 es_ES
dc.identifier.publicationfirstpage e0005644-1 es_ES
dc.identifier.publicationissue 5 es_ES
dc.identifier.publicationlastpage e0005644-26 es_ES
dc.identifier.publicationvolume 11 es_ES
dc.relation.projectID Gobierno de España. FISPI14/00366 es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/603181 en
dc.relation.projectID Gobierno de España. ISCIII-RETICRD16/0027/0008-FEDER es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en Reconocimiento es_ES
dc.rights.accessRights openAccess en
dc.authorUAM Requena Rolania, José María (259327)
dc.authorUAM Soto Álvarez, Manuel (260583)

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