miRNA profiling during antigendependent T cell activation: A role for miR-132-3p

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dc.contributor.author Gutiérrez-Vázquez, Cristina
dc.contributor.author Rodríguez-Galán, Ana
dc.contributor.author Fernández-Alfara, Marcos
dc.contributor.author Mittelbrunn, María
dc.contributor.author Sánchez-Cabo, Fátima
dc.contributor.author Martínez-Herrera, Dannys Jorge
dc.contributor.author Ramírez-Huesca, Marta
dc.contributor.author Pascual-Montano, Alberto
dc.contributor.author Sánchez-Madrid, Francisco
dc.contributor.other UAM. Departamento de Medicina es_ES
dc.contributor.other Instituto de Investigación del Hospital de La Princesa (IP) es_ES
dc.date.accessioned 2017-11-15T15:33:26Z
dc.date.available 2017-11-15T15:33:26Z
dc.date.issued 2017-06-14
dc.identifier.citation Scientific Reports 7 (2017): 3508 en_US
dc.identifier.issn 2045-2322 es_ES
dc.identifier.uri http://hdl.handle.net/10486/680259
dc.description.abstract microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3′UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells en_US
dc.description.sponsorship This work was supported by grants SAF2014-55579R from Ministerio de Economía y Competitividad-Spain, ERC-2011-AdG 294340-GENTRIS, CIBER CARDIOVASCULAR (FEDER and Instituto de Salud Carlos III), PIE-13-00041 and INDISNET S2011-BMD-2332 (F. S. M.). The Centro Nacional de Investigaciones Cardiovasculares (CNIC, Spain) is supported by the Ministerio de Economía y Competitividad-Spain and the Pro-CNIC Foundation en_US
dc.format.extent 8 pag. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Nature Publishing Group en_US
dc.relation.ispartof Scientific Reports en_US
dc.rights © 2017 The Author(s) en_US
dc.subject.other microRNAs (miRNAs) en_US
dc.subject.other T cell activation en_US
dc.subject.other Pik3r1 gene en_US
dc.subject.other MiR-132-3p en_US
dc.title miRNA profiling during antigendependent T cell activation: A role for miR-132-3p en_US
dc.type article en
dc.subject.eciencia Medicina es_ES
dc.relation.publisherversion http://dx.doi.org/10.1038/s41598-017-03689-7 es_ES
dc.identifier.doi 10.1038/s41598-017-03689-7 es_ES
dc.identifier.publicationfirstpage 3508-1 es_ES
dc.identifier.publicationissue 7 es_ES
dc.identifier.publicationlastpage 3508-8 es_ES
dc.relation.projectID Gobierno de España. SAF2014-55579R es_ES
dc.relation.projectID Gobierno de España. PIE-13-00041 es_ES
dc.relation.projectID Comunidad de Madrid. S2011-BMD-2332/INDISNET es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en
dc.rights.cc Reconocimiento es_ES
dc.rights.accessRights openAccess en
dc.authorUAM Mittelbrum Herrero, María (279535)
dc.authorUAM Sánchez Madrid, Francisco (259404)

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