Evaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model: An in vitro approach to the "race for the surface" theory

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dc.contributor.author Martínez-Pérez, M.
dc.contributor.author Pérez-Jorge, C.
dc.contributor.author Lozano, D.
dc.contributor.author Portal-Núñez, Sergio
dc.contributor.author Pérez-Tanoira, R.
dc.contributor.author Conde, A.
dc.contributor.author Arenas, M. A.
dc.contributor.author Hernández-López, J. M.
dc.contributor.author Damborenea, J. J. de
dc.contributor.author Gómez-Barrena, E.
dc.contributor.author Esbrit, P.
dc.contributor.author Esteban, J.
dc.contributor.other UAM. Departamento de Cirugía es_ES
dc.contributor.other UAM. Departamento de Medicina Preventiva y Salud Pública y Microbiología es_ES
dc.contributor.other Instituto de Investigación Sanitaria Fundación Jiménez Díaz (ISS-FJD) es_ES
dc.contributor.other Instituto de Investigación Sanitaria Hospital Universitario de La Paz (IdiPAZ) es_ES
dc.date.accessioned 2017-11-27T14:05:45Z
dc.date.available 2017-11-27T14:05:45Z
dc.date.issued 2017-05-01
dc.identifier.citation Bone & Joint Research 6.5 (2017): 315-322 en_US
dc.identifier.issn 2046-3758 es_ES
dc.identifier.uri http://hdl.handle.net/10486/680494
dc.description.abstract Objectives Implant-related infection is one of the most devastating complications in orthopaedic surgery. Many surface and/or material modifications have been developed in order to minimise this problem; however, most of the in vitro studies did not evaluate bacterial adhesion in the presence of eukaryotic cells, as stated by the 'race for the surface' theory. Moreover, the adherence of numerous clinical strains with different initial concentrations has not been studied. Methods We describe a method for the study of bacterial adherence in the presence of preosteoblastic cells. For this purpose we mixed different concentrations of bacterial cells from collection and clinical strains of staphylococci isolated from implant-related infections with preosteoblastic cells, and analysed the minimal concentration of bacteria able to colonise the surface of the material with image analysis. Results Our results show that clinical strains adhere to the material surface at lower concentrations than collection strains. A destructive effect of bacteria on preosteoblastic cells was also detected, especially with higher concentrations of bacteria. Conclusions The method described herein can be used to evaluate the effect of surface modifications on bacterial adherence more accurately than conventional monoculture studies. Clinical strains behave differently than collection strains with respect to bacterial adherence. en_US
dc.description.sponsorship This work was funded by the following grants from the Spanish MINECO (MAT2013- 48224-C2-2-R and MAT2013-48224-C2-1-R). M. Martínez-Pérez reports funding received from EFORT 2015 congress: travel supported by PFIZER, which is related to this article. J. Esteban and E. Gómez-Barrena report funding received from several companies for travel, expenses and grants, none of which is related to this article en_US
dc.format.extent 8 pag. es_ES
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher British Editorial Society of Bone and Joint Surgery en_US
dc.relation.ispartof Bone & Joint Research en_US
dc.rights © 2017 Martínez-Pérez et al. es_ES
dc.subject.other Bacterial adherence en_US
dc.subject.other Implant-related infection en_US
dc.subject.other Preosteoblastic cells en_US
dc.title Evaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model: An in vitro approach to the "race for the surface" theory en_US
dc.type article en
dc.subject.eciencia Medicina es_ES
dc.relation.publisherversion https://doi.org/10.1302/2046-3758.65.BJR- 2016-0226.R2 es_ES
dc.identifier.doi 10.1302/2046-3758.65.BJR- 2016-0226.R2 es_ES
dc.identifier.publicationfirstpage 315 es_ES
dc.identifier.publicationissue 5 es_ES
dc.identifier.publicationlastpage 322 es_ES
dc.identifier.publicationvolume 6 es_ES
dc.relation.projectID Gobierno de España. MAT2013- 48224-C2-2-R es_ES
dc.relation.projectID Gobierno de España. MAT2013-48224-C2-1-R es_ES
dc.type.version info:eu-repo/semantics/publishedVersion en
dc.rights.cc Reconocimiento – NoComercial es_ES
dc.rights.accessRights openAccess en

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