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dc.contributor.authorMartínez-Pérez, M.
dc.contributor.authorPérez-Jorge, C.
dc.contributor.authorLozano, D.
dc.contributor.authorPortal-Núñez, Sergio
dc.contributor.authorPérez-Tanoira, R.
dc.contributor.authorConde, A.
dc.contributor.authorArenas, M. A.
dc.contributor.authorHernández-López, J. M.
dc.contributor.authorDamborenea, J. J. de
dc.contributor.authorGómez-Barrena, E.
dc.contributor.authorEsbrit, P.
dc.contributor.authorEsteban, J.
dc.contributor.otherUAM. Departamento de Cirugíaes_ES
dc.contributor.otherUAM. Departamento de Medicina Preventiva y Salud Pública y Microbiologíaes_ES
dc.contributor.otherInstituto de Investigación Sanitaria Fundación Jiménez Díaz (IIS-FJD)es_ES
dc.contributor.otherInstituto de Investigación Sanitaria Hospital Universitario de La Paz (IdiPAZ)es_ES
dc.date.accessioned2017-11-27T14:05:45Z
dc.date.available2017-11-27T14:05:45Z
dc.date.issued2017-05-01
dc.identifier.citationBone & Joint Research 6.5 (2017): 315-322en_US
dc.identifier.issn2046-3758es_ES
dc.identifier.urihttp://hdl.handle.net/10486/680494
dc.description.abstractObjectives Implant-related infection is one of the most devastating complications in orthopaedic surgery. Many surface and/or material modifications have been developed in order to minimise this problem; however, most of the in vitro studies did not evaluate bacterial adhesion in the presence of eukaryotic cells, as stated by the 'race for the surface' theory. Moreover, the adherence of numerous clinical strains with different initial concentrations has not been studied. Methods We describe a method for the study of bacterial adherence in the presence of preosteoblastic cells. For this purpose we mixed different concentrations of bacterial cells from collection and clinical strains of staphylococci isolated from implant-related infections with preosteoblastic cells, and analysed the minimal concentration of bacteria able to colonise the surface of the material with image analysis. Results Our results show that clinical strains adhere to the material surface at lower concentrations than collection strains. A destructive effect of bacteria on preosteoblastic cells was also detected, especially with higher concentrations of bacteria. Conclusions The method described herein can be used to evaluate the effect of surface modifications on bacterial adherence more accurately than conventional monoculture studies. Clinical strains behave differently than collection strains with respect to bacterial adherence.en_US
dc.description.sponsorshipThis work was funded by the following grants from the Spanish MINECO (MAT2013- 48224-C2-2-R and MAT2013-48224-C2-1-R). M. Martínez-Pérez reports funding received from EFORT 2015 congress: travel supported by PFIZER, which is related to this article. J. Esteban and E. Gómez-Barrena report funding received from several companies for travel, expenses and grants, none of which is related to this articleen_US
dc.format.extent8 pag.es_ES
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherBritish Editorial Society of Bone and Joint Surgeryen_US
dc.relation.ispartofBone & Joint Researchen_US
dc.rights© 2017 Martínez-Pérez et al.es_ES
dc.subject.otherBacterial adherenceen_US
dc.subject.otherImplant-related infectionen_US
dc.subject.otherPreosteoblastic cellsen_US
dc.titleEvaluation of bacterial adherence of clinical isolates of Staphylococcus sp. using a competitive model: An in vitro approach to the "race for the surface" theoryen_US
dc.typearticleen
dc.subject.ecienciaMedicinaes_ES
dc.relation.publisherversionhttps://doi.org/10.1302/2046-3758.65.BJR- 2016-0226.R2es_ES
dc.identifier.doi10.1302/2046-3758.65.BJR- 2016-0226.R2es_ES
dc.identifier.publicationfirstpage315es_ES
dc.identifier.publicationissue5es_ES
dc.identifier.publicationlastpage322es_ES
dc.identifier.publicationvolume6es_ES
dc.relation.projectIDGobierno de España. MAT2013- 48224-C2-2-Res_ES
dc.relation.projectIDGobierno de España. MAT2013-48224-C2-1-Res_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.rights.ccReconocimiento – NoComerciales_ES
dc.rights.accessRightsopenAccessen
dc.facultadUAMFacultad de Medicina
dc.institutoUAMInstituto de Investigación Sanitaria Fundación Jiménez Díaz (ISS-FJD)
dc.institutoUAMInstituto de Investigación Sanitaria Hospital Universitario de La Paz (IdiPAZ)


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