dc.contributor.author | Nayernia, Zeynab | |
dc.contributor.author | Colaianna, Marilena | |
dc.contributor.author | Robledinos-Antón, Natalia | |
dc.contributor.author | Gutzwiller, Eveline | |
dc.contributor.author | Sloan-Béna, Frédérique | |
dc.contributor.author | Stathaki, Elisavet | |
dc.contributor.author | Hibaoui, Yousef | |
dc.contributor.author | Cuadrado Pastor, Antonio | |
dc.contributor.author | Hescheler, Jürgen | |
dc.contributor.author | Stasia, Marie-José | |
dc.contributor.author | Saric, Tomo | |
dc.contributor.author | Jaquet, Vincent | |
dc.contributor.author | Krause, Karl Heinz | |
dc.contributor.other | UAM. Departamento de Bioquímica | es_ES |
dc.contributor.other | Instituto de Investigaciones Biomédicas "Alberto Sols" (IIBM) | es_ES |
dc.date.accessioned | 2017-11-29T15:43:56Z | |
dc.date.available | 2017-11-29T15:43:56Z | |
dc.date.issued | 2017-04-24 | |
dc.identifier.citation | Redox Biology 13 (2017): 82-93 | en_US |
dc.identifier.issn | 2213-2317 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10486/680546 | |
dc.description.abstract | There is emerging evidence for the involvement of reactive oxygen species (ROS) in the regulation of stem cells
and cellular differentiation. Absence of the ROS-generating NADPH oxidase NOX2 in chronic granulomatous
disease (CGD) patients, predominantly manifests as immune deficiency, but has also been associated with
decreased cognition. Here, we investigate the role of NOX enzymes in neuronal homeostasis in adult mouse brain
and in neural cells derived from human induced pluripotent stem cells (iPSC). High levels of NOX2 were found in
mouse adult neurogenic regions. In NOX2-deficient mice, neurogenic regions showed diminished redox
modifications, as well as decrease in neuroprecursor numbers and in expression of genes involved in neural
differentiation including NES, BDNF and OTX2. iPSC from healthy subjects and patients with CGD were used to
study the role of NOX2 in human in vitro neuronal development. Expression of NOX2 was low in
undifferentiated iPSC, upregulated upon neural induction, and disappeared during neuronal differentiation. In
human neurospheres, NOX2 protein and ROS generation were polarized within the inner cell layer of rosette
structures. NOX2 deficiency in CGD-iPSCs resulted in an abnormal neural induction in vitro, as revealed by a
reduced expression of neuroprogenitor markers (NES, BDNF, OTX2, NRSF/REST), and a decreased generation of
mature neurons. Vector-mediated NOX2 expression in NOX2-deficient iPSCs rescued neurogenesis. Taken
together, our study provides novel evidence for a regulatory role of NOX2 during early stages of neurogenesis in
mouse and human | en_US |
dc.description.sponsorship | This study was supported by the Swiss National Foundation (SNF) grant number 31003A-160220/1, European Community's Framework Programme (FP7/2007–2013) under grant 278611 (Neurinox), Swiss State Secretariat for Education and Innovation C13.0142, German Federal Ministry for Education and Research (Bundesministerium (für Bildung
und Forschung, BMBF; grant number 01GN0824), Ministry for Innovation, Science und Research of the State of North Rhine-Westphalia (323-400-010-13), and Köln Fortune Programs of the Medical Faculty of the University of Cologne, Germany. Natalia
Robledinos-Anton was supported by a grant from the COST action EUROS BM1203 and the program for training university lecturers (FPU14/01073) from Spanish Ministry of Education | en_US |
dc.format.extent | 12 pag. | es_ES |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | Elsevier B.V. | en_US |
dc.relation.ispartof | Redox Biology | en_US |
dc.rights | © 2017 The Authors | en_US |
dc.subject.other | NOX2 | en_US |
dc.subject.other | Reactive oxygen species | en_US |
dc.subject.other | Adult neurogenesis in vitro neural differentiation | en_US |
dc.subject.other | Neural stem/progenitor cells | en_US |
dc.subject.other | Induced pluripotent stem cells (iPSC) | en_US |
dc.title | Decreased neural precursor cell pool in NADPH oxidase 2-deficiency: From mouse brain to neural differentiation of patient derived iPSC | en_US |
dc.type | article | en |
dc.subject.eciencia | Medicina | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1016/j.redox.2017.04.026 | en |
dc.identifier.doi | 10.1016/j.redox.2017.04.026 | es_ES |
dc.identifier.publicationfirstpage | 82 | es_ES |
dc.identifier.publicationlastpage | 93 | es_ES |
dc.identifier.publicationvolume | 13 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EC/H2020/1203/EU//COST | en |
dc.relation.projectID | info:eu-repo/grant/Agreement/EC/FP7/278611 | en |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.rights.cc | Reconocimiento – NoComercial – SinObraDerivada | es_ES |
dc.rights.accessRights | openAccess | en |
dc.authorUAM | Robledinos Antón, Natalia (271160) | |
dc.facultadUAM | Facultad de Medicina | |
dc.institutoUAM | Instituto de Investigaciones Biomédicas "Alberto Sols" (IIBM) | |