Resequencing of the Leishmania infantum (strain JPCM5) genome and de novo assembly into 36 contigs
EntityUAM. Departamento de Biología Molecular; Centro de Biología Molecular Severo Ochoa (CBM)
PublisherNature Publishing Group
10.1038/s41598-017-18374-yScientific Reports 7 (2017): 18050
Funded byThis work was supported by grants from Proyecto del Ministerio de Economía y Competitividad (SAF2013-47556-R, co-financed with FEDER funds), and the Fondo de Investigaciones Sanitarias (ISCIII-RETIC RD16/0027/0008-FEDER). The CBMSO receives institutional grants from the Fundación Ramón Areces and from the Fundación Banco de Santander
ProjectGobierno de España. SAF2013-47556-R; Gobierno de España. ISCIII-RETIC RD16/0027/0008-FEDER
SubjectsLeishmania parasites; Infantum; 36 contigs; Robust assembly; Biología y Biomedicina / Biología
Rights© The Author(s) 2017
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
Leishmania parasites are the causative of leishmaniasis, a group of potentially fatal human diseases. Control strategies for leishmaniasis can be enhanced by genome based investigations. The publication in 2005 of the Leishmania major genome sequence, and two years later the genomes for the species Leishmania braziliensis and Leishmania infantum were major milestones. Since then, the L. infantum genome, although highly fragmented and incomplete, has been used widely as the reference genome to address whole transcriptomics and proteomics studies. Here, we report the sequencing of the L. infantum genome by two NGS methodologies and, as a result, the complete genome assembly on 36 contigs (chromosomes). Regarding the present L. infantum genome-draft, 495 new genes have been annotated, a hundred have been corrected and 75 previous annotated genes have been discontinued. These changes are not only the result of an increase in the genome size, but a significant contribution derives from the existence of a large number of incorrectly assembled regions in current chromosomal scaffolds. Furthermore, an improved assembly of tandemly repeated genes has been obtained. All these analyses support that the de novo assembled L. infantum genome represents a robust assembly and should replace the currently available in the databases
Google Scholar:González-de la Fuente, Sandra - Peiró-Pastor, Ramón - Rastrojo, Alberto - Moreno, Javier - Carrasco-Ramiro, Fernando - Requena Rolania, José María - Aguado, Begoña
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