UAM_Biblioteca | Unified search engine | Scientific Production Portal | UAM Research Data Repository
Universidad Autónoma de Madrid
Biblos-e Archivo
We are onFacebookCanal BiblosYouTubeTwitterPinterestWhatsappInstagram
    • español
    • English
  • English 
    • español
    • English
  • Log in
JavaScript is disabled for your browser. Some features of this site may not work without it.

Search Biblos-e Archivo

Advanced Search

Browse

All of Biblos-e ArchivoCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsFacultiesThis CollectionBy Issue DateAuthorsTitlesSubjectsFaculties

My Account

Log inRegister

Statistics

View Usage Statistics

Help

Information about Biblos-e ArchivoI want to submit my workI want to submit my doctoral thesisFrequently Asked QuestionsCopyrightsFinancial Agencies and OA policy

UAM_Biblioteca

View Item 
  •   Biblos-e Archivo
  • 1 - Producción científica en acceso abierto de la UAM
  • Producción científica en acceso abierto de la UAM
  • View Item
  •   Biblos-e Archivo
  • 1 - Producción científica en acceso abierto de la UAM
  • Producción científica en acceso abierto de la UAM
  • View Item

Parathyroid hormone-related protein exhibits antioxidant features in osteoblastic cells through its N-terminal and osteostatin domains

Author
Portal-Núñez, Sergio; Ardura, J. A.; Lozano, D.; Martínez de Toda, I.; De La Fuente, M.; Herrero-Beaumont, Gabriel; Largo, R.; Esbrit, P.
Entity
UAM. Departamento de Medicina; Instituto de Investigación Sanitaria Fundación Jiménez Díaz (IIS-FJD)
Publisher
British Editorial Society of Bone and Joint Surgery
Date
2018-01-01
Citation
10.1302/2046-3758.71.BJR-2016-0242.R2
Bone Joint Research 7.1 (2018): 58-68
 
 
 
ISSN
2046-3758
DOI
10.1302/2046-3758.71.BJR-2016-0242.R2
Funded by
This work has been funded by grants from the Fundacion para la Investigacion Osea y Metabolismo Mineral-FEIOMM and the Instituto de Salud Carlos III (PI11/00449, PI15/00340, PI1600065, RD12/0043/0029, RD12/0043/0008 and RD12/0043/0018,). J. A. Ardua, D. Lozano, and S. Portal-Nunez are recipients of postdoctoral contracts from the Ministerio de Economia y Competitividad, Juan de la Cierva program JCI-2011-09548, FPDI-2013-17268, and RETICEF [FEDER “una manera de hacer Europa” (RD12/0043/0008)]
Project
Gobierno de España. PI11/00449; Gobierno de España. PI15/00340; Gobierno de España. PI1600065; Gobierno de España. RD12/0043/0029; Gobierno de España. RD12/0043/0008; Gobierno de España. RD12/0043/0018
Editor's Version
https://doi.org/10.1302/2046-3758.71.BJR-2016-0242.R2
Subjects
Antioxidant activity; Osteostatin; Parathyroid hormone-related protein; Medicina
URI
http://hdl.handle.net/10486/681580
Rights
© 2018 Portal-Núñez et al.

Licencia de Creative Commons
Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial 4.0 Internacional.

Abstract

Objectives: Oxidative stress plays a major role in the onset and progression of involutional osteoporosis. However, classical antioxidants fail to restore osteoblast function. Interestingly, the bone anabolism of parathyroid hormone (PTH) has been shown to be associated with its ability to counteract oxidative stress in osteoblasts. The PTH counterpart in bone, which is the PTHrelated protein (PTHrP), displays osteogenic actions through both its N-terminal PTH-like region and the C-terminal domain. Methods: We examined and compared the antioxidant capacity of PTHrP (1-37) with the C-terminal PTHrP domain comprising the 107-111 epitope (osteostatin) in both murine osteoblastic MC3T3-E1 cells and primary human osteoblastic cells. Results: We showed that both N- and C-terminal PTHrP peptides at 100 nM decreased reactive oxygen species production and forkhead box protein O activation following hydrogen peroxide (H 2 O 2 )-induced oxidation, which was related to decreased lipid oxidative damage and caspase-3 activation in these cells. This was associated with their ability to restore the deleterious effects of H 2 O 2 on cell growth and alkaline phosphatase activity, as well as on the expression of various osteoblast differentiation genes. The addition of Rp-cyclic 3',5'-hydrogen phosphorothioate adenosine triethylammonium salt (a cyclic 3',5'-adenosine monophosphate antagonist) and calphostin C (a protein kinase C inhibitor), or a PTH type 1 receptor antagonist, abrogated the effects of N-terminal PTHrP, whereas protein phosphatase 1 (an Src kinase activity inhibitor), SU1498 (a vascular endothelial growth factor receptor 2 inhibitor), or an anti osteostatin antiserum, inhibited the effects of C-terminal PTHrP. Conclusion: These findings indicate that the antioxidant properties of PTHrP act through its N- and C-terminal domains and provide novel insights into the osteogenic action of PTHrP.
Show full item record

Files in this item

Thumbnail
Name
parathyroid_portal_bjr_2018.pdf
Size
1.103Mb
Format
PDF

Refworks Export

Delicious Save this on Delicious

Google™ Scholar:Portal-Núñez, Sergio - Ardura, J. A. - Lozano, D. - Martínez de Toda, I. - De La Fuente, M. - Herrero-Beaumont, Gabriel - Largo, R. - Esbrit, P.

This item appears in the following Collection(s)

  • Producción científica en acceso abierto de la UAM [15094]

Related items

Showing items related by title, author, creator and subject.

  • Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy 

    Estañ, María Cristina; Fernández-Núñez, Elisa; Zaki, Maha S.; Esteban, María Isabel; Donkervoort, Sandra; Hawkins, Cynthia; Caparros-Martin, José A.; Saade, Dimah; Hu, Ying; Bolduc, Véronique; Chao, Katherine Ru Yui; Nevado, Julián; Lamuedra, Ana; Largo, Raquel; Herrero-Beaumont, Gabriel; Regadera, Javier; Hernandez-Chico, Concepción; Tizzano, Eduardo F.; Martinez-Glez, Victor; Carvajal, Jaime J.; Zong, Ruiting; Nelson, David L.; Otaify, Ghada A.; Temtamy, Samia; Aglan, Mona; Issa, Mahmoud; Bönnemann, Carsten G.; Lapunzina, Pablo; Yoon, Grace; Ruiz-Perez, Victor L.
    2019
  • Inflamación sinovial en un modelo experimental de síndrome metabólico en conejo 

    Martínez-Calatrava, M. J.; Largo, R.; Prieto-Potín, I.; Herrero-Beaumont, Gabriel
    2011
  • Hypercholesterolemia boosts joint destruction in chronic arthritis. An experimental model aggravated by foam macrophage infiltration 

    Prieto-Potín, Ivan; Roman-Blas, Jorge A.; Martínez Calatrava, María José; Gómez, Rodolfo; Largo, Raquel; Herrero-Beaumont, Gabriel
    2013-08-13
All the documents from Biblos-e Archivo are protected by copyrights. Some rights reserved.
Universidad Autónoma de Madrid. Biblioteca
Contact Us | Send Feedback
We are onFacebookCanal BiblosYouTubeTwitterPinterestWhatsappInstagram
 

 

All the documents from Biblos-e Archivo are protected by copyrights. Some rights reserved.
Universidad Autónoma de Madrid. Biblioteca
Contact Us | Send Feedback
We are onFacebookCanal BiblosYouTubeTwitterPinterestWhatsappInstagram