Gene expression analyses determine two different subpopulations in KIT-negative GIST-like (KNGL) patients
dc.contributor.author | Moura, David S. | |
dc.contributor.author | Ramos, Rafael | |
dc.contributor.author | Fernández-Serra, Antonio | |
dc.contributor.author | Serrano, Teresa | |
dc.contributor.author | Cruz, Julia | |
dc.contributor.author | Álvarez-Alegret, Ramiro | |
dc.contributor.author | Ortiz-Durán, Rosa | |
dc.contributor.author | Vicioso, Luis | |
dc.contributor.author | Gómez-Dorronsoro, María Luisa | |
dc.contributor.author | Garcia del Muro, Xavier | |
dc.contributor.author | Martínez-Trufero, Javier | |
dc.contributor.author | Rubio-Casadevall, Jordi | |
dc.contributor.author | Sevilla, Isabel | |
dc.contributor.author | Laínez, Nuria | |
dc.contributor.author | Gutiérrez, Antonio | |
dc.contributor.author | Serrano, César | |
dc.contributor.author | López-Álvarez, María | |
dc.contributor.author | Hindi, Nadia | |
dc.contributor.author | Tarón, Miguel | |
dc.contributor.author | López Guerrero, José Antonio | |
dc.contributor.author | Martín-Broto, Javier | |
dc.contributor.other | UAM. Departamento de Biología Molecular | es_ES |
dc.date.accessioned | 2018-06-22T17:33:25Z | |
dc.date.available | 2018-06-22T17:33:25Z | |
dc.date.issued | 2018-04-03 | |
dc.identifier.citation | Oncotarget 9.25 (2018): 17576-17588 | en_US |
dc.identifier.issn | 1949-2553 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10486/683266 | |
dc.description.abstract | Introduction: There are limited findings available on KIT-negative GIST-like (KNGL) population. Also, KIT expression may be post-transcriptionally regulated by miRNA221 and miRNA222. Hence, the aim of this study is to characterize KNGL population, by differential gene expression, and to analyze miRNA221/222 expression and their prognostic value in KNGL patients. Methods: KIT, PDGFRA, DOG1, IGF1R, MIR221 and MIR222 expression levels were determined by qRT-PCR. We also analyzed KIT and PDGFRA mutations, DOG1 expression, by immunohistochemistry, along with clinical and pathological data. Disease-free survival (DFS) and overall survival (OS) differences were calculated using Log-rank test. Results: Hierarchical cluster analyses from gene expression data identified two groups: group I had KIT, DOG1 and PDGFRA overexpression and IGF1R underexpression and group II had overexpression of IGF1R and low expression of KIT, DOG1 and PDGFRA. Group II had a significant worse OS (p = 0.013) in all the series, and showed a tendency for worse OS (p = 0.11), when analyzed only the localized cases. MiRNA222 expression was significantly lower in a control subset of KIT-positive GIST (p < 0.001). OS was significantly worse in KNGL cases with higher expression of MIR221 (p = 0.028) or MIR222 (p = 0.014). Conclusions: We identified two distinct KNGL subsets, with a different prognostic value. Increased levels of miRNA221/222, which are associated with worse OS, could explain the absence of KIT protein expression of most KNGL tumors | en_US |
dc.description.sponsorship | The project was funded by the Instituto de Salud Carlos III (ISCIII) - Fondo Europeo de Desarrollo Regional (FEDER), through a public competitive call (project reference PI15/01254; principal Investigator Javier Martín Broto), and the Spanish Group for Research on Sarcoma (GEIS) | en_US |
dc.format.extent | 13 pag. | es_ES |
dc.format.mimetype | application/pdf | en |
dc.language.iso | eng | en |
dc.publisher | Impact Journals | en_US |
dc.relation.ispartof | Oncotarget | en_US |
dc.rights | © 2018 Moura et al. | es_ES |
dc.subject.other | KNGL | en_US |
dc.subject.other | miRNA221/222 cluster | en_US |
dc.subject.other | KIT | en_US |
dc.subject.other | DOG1 | en_US |
dc.subject.other | IGF1R | en_US |
dc.title | Gene expression analyses determine two different subpopulations in KIT-negative GIST-like (KNGL) patients | en_US |
dc.type | article | en |
dc.subject.eciencia | Biología y Biomedicina / Biología | es_ES |
dc.relation.publisherversion | https://doi.org/10.18632/oncotarget.24799 | es_ES |
dc.identifier.doi | 10.18632/oncotarget.24799 | es_ES |
dc.identifier.publicationfirstpage | 17576 | es_ES |
dc.identifier.publicationissue | 25 | es_ES |
dc.identifier.publicationlastpage | 17588 | es_ES |
dc.identifier.publicationvolume | 9 | es_ES |
dc.relation.projectID | Gobierno de España. PI15/01254 | es_ES |
dc.type.version | info:eu-repo/semantics/publishedVersion | en |
dc.rights.cc | Reconocimiento | es_ES |
dc.rights.accessRights | openAccess | en |
dc.facultadUAM | Facultad de Ciencias |