Primer-Independent DNA Synthesis by a Family B DNA Polymerase from Self-Replicating Mobile Genetic Elements
EntityUAM. Departamento de Bioquímica; Centro de Biología Molecular Severo Ochoa (CBM)
PublisherElsevier (Cell Press)
10.1016/j.celrep.2017.10.039Cell Reports 21.6 (2018): 1575–1587
Funded byM.S.’s lab is funded by the Spanish Ministry of Economy and Competitiveness (BFU2014-52656P). M.R.-R. was supported by a ComFuturo Grant (NewPols4Biotech) from Fundación General CSIC. C.D.O. was holder of a ‘‘Plan de Empleo Juvenil’’ contract from Madrid Regional Government (funded by YEI program from European Social Fund, EC). An institutional grant from Fundación Ramón Areces to the Centro de Biología Molecular Severo Ochoa is also acknowledged. P.F. was funded by the European Research Council under the European Union’s Seventh Framework Program (FP/2007-2013)/ Project EVOMOBIL - ERC Grant Agreement 340440.
ProjectGobierno de España. BFU2014-52656P
SubjectsDe novo DNA synthesis; DNA damage; DNA replication; Family B DNA polymerase; Primer-independent DNA synthesis; Self-replicating mobile element; Translesion synthesis; Medicina
Rights© 2017 The Authors
Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Family B DNA polymerases (PolBs) play a central role during replication of viral and cellular chromosomes. Here, we report the discovery of a third major group of PolBs, which we denote primer-independent PolB (piPolB), that might be a link between the previously known protein-primed and RNA/DNA-primed PolBs. PiPolBs are encoded by highly diverse mobile genetic elements, pipolins, integrated in the genomes of diverse bacteria and also present as circular plasmids in mitochondria. Biochemical characterization showed that piPolB displays efficient DNA polymerization activity that can use undamaged and damaged templates and is endowed with proofreading and strand displacement capacities. Remarkably, the protein is also capable of template-dependent de novo DNA synthesis, i.e., DNA-priming activity, thereby breaking the long-standing dogma that replicative DNA polymerases require a pre-existing primer for DNA synthesis. We suggest that piPolBs are involved in self-replication of pipolins and may also contribute to bacterial DNA damage tolerance. Redrejo-Rodríguez et al. report and characterize a DNA polymerase group (piPolB) from the B family that can perform primer-independent DNA replication. PiPolBs are encoded by Pipolins, diverse self-replicating genetic elements that are widespread among bacterial phyla and in mitochondria.
Google Scholar:Redrejo Rodríguez, Modesto - Ordóñez, Carlos D. - Berjón-Otero, Mónica - Moreno-González, Juan - Aparicio-Maldonado, Cristian - Forterre, Patrick - Salas, Margarita - Krupovic, Mart
This item appears in the following Collection(s)
Showing items related by title, author, creator and subject.
López-Rodríguez, Rosario; Hernández-Bartolomé, Ángel; Borque, María Jesús; Rodríguez-Muñoz, Yolanda; Martín-Vílchez, Samuel; García-Buey, Luisa; González-Moreno, Leticia; Real-Martínez, Yolanda; Muñoz de Rueda, Paloma; Salmerón, Javier; Vidal-Castiñeira, José Ramón; López-Larrea, Carlos; Rodrigo, Luis; Moreno-Otero, Ricardo; Sanz-Cameno, Paloma
Ordoñez Cencerrado, Carlos David