Caveolin-1α regulates primary cilium length by controlling RhoA GTPase activity
EntityUAM. Departamento de Biología
10.1038/s41598-018-38020-5Scientific Reports 9 (2019): 1116
Funded byThis work was supported by grants B2017/BMD-3817 from the Comunidad de Madrid and BFU2015-67266-R from the Spanish Ministerio de Economía y Competitividad/Fondo Europeo de Desarrollo Regional (MINECO/FEDER) to IC and MAA, respectively. We also acknowledge the Micro and Nanofabrication Laboratory of the Instituto de Micro y Nanotecnología, which is funded from the Comunidad de Madrid (project SpaceTec, S2013/ICE2822), MINECO (project CSIC13-4E-1794) and EU (FEDER, FSE), for invaluable help on SEM. Contracts from the Ministerio de Educación, Cultura y Deporte (JF-B, JC-A) and the MINECO (MB-R) are also acknowledged
ProjectGobierno de España. CSIC13-4E-1794; Comunidad de Madrid. S2013/ICE-2822/SPACETEC-CM
Subjectscilia; flagella; intraflagellar transport; Biología y Biomedicina / Biología
Rights© 2019, The Author(s)
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
The primary cilium is a single non-motile protrusion of the plasma membrane of most types of mammalian cell. The structure, length and function of the primary cilium must be tightly controlled because their dysfunction is associated with disease. Caveolin 1 (Cav1), which is best known as a component of membrane invaginations called caveolae, is also present in non-caveolar membrane domains whose function is beginning to be understood. We show that silencing of α and β Cav1 isoforms in different cell lines increases ciliary length regardless of the route of primary ciliogenesis. The sole expression of Cav1α, which is distributed at the apical membrane, restores normal cilium size in Cav1 KO MDCK cells. Cells KO for only Cav1α, which also show long cilia, have a disrupted actin cytoskeleton and reduced RhoA GTPase activity at the apical membrane, and a greater accumulation of Rab11 vesicles at the centrosome. Subsequent experiments showed that DIA1 and ROCK help regulate ciliary length. Since MDCK cells lack apical caveolae, our results imply that non-caveolar apical Cav1α is an important regulator of ciliary length, exerting its effect via RhoA and its effectors, ROCK and DIA1
Google Scholar:Rangel, Laura - Bernabé-Rubio, Miguel - Fernández-Barrera, Jaime - Casares-Arias, Javier - Millán, Jaime - Alonso, Miguel Angel - Correas Hornero, María Isabel
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Casares-Arias, Javier; Ujue Gonzalez, María; San Paulo, Alvaro; Ventimiglia, Leandro N.; Sadler, Jessica B.A.; Miguez Gómez, David; Labat-de-Hoz, Leticia; Rubio-Ramos, Armando; Rangel, Laura; Bernabe-Rubio, Miguel; Fernandez-Barrera, Jaime; Correas Hornero, María Isabel; Martın-Serrano, Juan; Alonso, Miguel A.