Fluorescent in vivo imaging of reactive oxygen species and redox potential in plants

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dc.contributor.author Ortega-Villasante, Cristina
dc.contributor.author Burén, Stefan
dc.contributor.author Blázquez-Castro, Alfonso
dc.contributor.author Barón-Sola, Ángel
dc.contributor.author Hernández, Luis E.
dc.contributor.other UAM. Departamento de Biología es_ES
dc.contributor.other UAM. Departamento de Física de Materiales es_ES
dc.date.accessioned 2019-10-07T07:38:29Z
dc.date.available 2019-10-07T07:38:29Z
dc.date.issued 2018-07-01
dc.identifier.citation Free Radical Biology and Medicine 122 (2018): 202-220 en_US
dc.identifier.issn 1873-4596 (online) en_US
dc.identifier.issn 0891-5849 (print) en_US
dc.identifier.uri http://hdl.handle.net/10486/688783
dc.description.abstract Reactive oxygen species (ROS) are by-products of aerobic metabolism, and excessive production can result in oxidative stress and cell damage. In addition, ROS function as cellular messengers, working as redox regulators in a multitude of biological processes. Understanding ROS signalling and stress responses requires methods for precise imaging and quantification to monitor local, subcellular and global ROS dynamics with high selectivity, sensitivity and spatiotemporal resolution. In this review, we summarize the present knowledge for in vivo plant ROS imaging and detection, using both chemical probes and fluorescent protein-based biosensors. Certain characteristics of plant tissues, for example high background autofluorescence in photosynthetic organs and the multitude of endogenous antioxidants, can interfere with ROS and redox potential detection, making imaging extra challenging. Novel methods and techniques to measure in vivo plant ROS and redox changes with better selectivity, accuracy, and spatiotemporal resolution are therefore desirable to fully acknowledge the remarkably complex plant ROS signalling networks en_US
dc.description.sponsorship This work was funded by a grant from the Spanish Ministry of Economy and Competitiveness ( AGL2014–53771-R ). Alfonso Blázquez-Castro acknowledges funding under the Marie Skłodowska-Curie Action COFUND 2015 (EU project 713366 – InterTalentum) en_US
dc.format.extent 53 pag. en_US
dc.format.mimetype application/pdf en
dc.language.iso eng en
dc.publisher Elsevier en_US
dc.relation.ispartof Free Radical Biology and Medicine en_US
dc.rights © 2018 Elsevier Inc. en_US
dc.subject.other Fluorescent probes en_US
dc.subject.other Plant en_US
dc.subject.other Protein biosensors en_US
dc.subject.other Reactive Oxygen Species (ROS) en_US
dc.subject.other Redox signalling en_US
dc.subject.other In vivo detection en_US
dc.title Fluorescent in vivo imaging of reactive oxygen species and redox potential in plants en_US
dc.type article en
dc.subject.eciencia Botánica en_US
dc.date.embargoend 2019-07-01
dc.relation.publisherversion https://doi.org/10.1016/j.freeradbiomed.2018.04.005 es_ES
dc.identifier.doi 10.1016/j.freeradbiomed.2018.04.005 es_ES
dc.identifier.publicationfirstpage 202 es_ES
dc.identifier.publicationlastpage 220 es_ES
dc.identifier.publicationvolume 122 es_ES
dc.relation.projectID Gobierno de España. AGL2014–53771-R es_ES
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/713366/EU//InterTalentum en_US
dc.type.version info:eu-repo/semantics/acceptedVersion en
dc.rights.cc Reconocimiento – NoComercial – SinObraDerivada es_ES
dc.rights.accessRights openAccess en
dc.authorUAM Ortega Villasante, Cristina (260482)
dc.authorUAM Blázquez Castro, Alfonso (263213)
dc.authorUAM Barón Sola, Ángel (263907)
dc.authorUAM Hernández Rodríguez, Luis Eduardo (258434)

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