Carbon nanodots based biosensors for gene mutation detection
EntityUAM. Departamento de Química Analítica y Análisis Instrumental
10.1016/j.snb.2017.10.105Sensors and Actuators, B: Chemical 256 (2018): 226-233
ISSN1873-3077 (online); 0925-4005 (print)
Funded byThis work has been supported by the Comunidad Autónoma de Madrid NANOAVANSENS (project No. S2013/MIT-3029) and Spanish Ministerio de Economía, Industria y Competitividad through projects Nos. CTQ2015-71955-REDT (ELECTROBIONET) and CTQ2014-53334-C2-1-R. E. L. thanks the Fulbright scholarship-Salvador de Madariaga program from Spanish Ministerio de Economía, Industria y Competitividad. I. B. gratefully acknowledges the FPI-2012 Grant from Spanish Ministerio de Economía y Competitividad. R.W. gratefully acknowledges support by the European Union structural funds and the Comunidad de Madrid MAD2D-CM Program (S2013/MIT-3007), as well as by the Spanish Ministerio de Economía, Industria y Competitividad through project No. MAT2015-71879-P
ProjectComunidad de Madrid. S2013/MIT-3029/NANOAVANSENS; Gobierno de España. CTQ2015-71955-REDT; Gobierno de España. CTQ2014-53334-C2-1-R; Comunidad de Madrid. S2013/MIT‐3007/MAD2D; Gobierno de España. MAT2015-71879-P
SubjectsCarbon nanodots; Detection of CFTR gene mutation; DNA electrochemical biosensor; Química
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Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
An electrochemical DNA biosensor based on a carbon nanodots (CDs) modified screen-printed gold electrode as a transducer is reported in this work. CDs were synthesized by thermal carbonization of ethyleneglycol bis-(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) and characterized by different techniques (DLS, TEM, FTIR, Raman). The electrode surface modification was accomplished by drop-casting a suspension of CDs. SEM analysis and cyclic voltammetry were used to characterize the resulting modified electrode. Synthetic 25-mer or 100-mer DNA capture probes, capable to hybridize with a specific sequence of the pathogen Helicobacter pylori or the cystic fibrosis transmembrane regulator (CFTR) gene were attached to the CDs-gold surface. A 25-bases synthetic fully complementary sequence or a single nucleotide polymorphism to the DNA capture probe and a 373-bases PCR amplicon of exon 11 of CFTR containing a sequence complementary to the capture probe, were employed as target. The hybridization event was electrochemically monitored by using safranine as redox indicator, which selectively binds to double stranded DNA (dsDNA). A detection limit of 0.16 nM was obtained for the 25-mer synthetic target DNA. The biosensor shows a very high reproducibility and selectivity, allowing to detect a single nucleotide polymorphism. It has been applied to the detection of F508del mutation in the CFTR gene
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Google Scholar:García-Mendiola, Tania - Bravo, Iria - López-Moreno, José María - Pariente Alonso, Félix - Wannemacher, Reinhold - Weber, Karina - Popp, Jürgen - Lorenzo, Encarnación
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