Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations

García Mendiola, Tania; Elosegui, Cristina Garcia; Bravo, Iria; Pariente Alonso, Félix; Jacobo-Martin, Alejandra; Navio, Cristina; Rodriguez, Isabel; Wannemacher, Reinhold; Lorenzo Abad, Encarnación

UAM_Biblioteca

Author

García Mendiola, Tania

; Elosegui, Cristina Garcia; Bravo, Iria; Pariente Alonso, Félix

; Jacobo-Martin, Alejandra; Navio, Cristina; Rodriguez, Isabel; Wannemacher, Reinhold; Lorenzo Abad, Encarnación

Entity

UAM. Departamento de Química Analítica y Análisis Instrumental

Publisher

Springer Verlag

Date

2019-05-01

Citation

Microchimica Acta 186.5 (2019): 293

ISSN

1436-5073 (online); 0026-3672 (print)

DOI

10.1007/s00604-019-3386-9

Funded by

CAM projects: TRANSNANOAVANSENS-CM (S2018/NMT-4349) and MAD2D-CM Program. MEIC projects: CTQ2017-84309-C2-1-R and MAT2015-71879-P. We thank the Confocal Microscopy and Flow Cytometry Services of CBMSO

Project

Comunidad de Madrid. S2013/MIT‐3007/MAD2D; Comunidad de Madrid. S2018/NMT-4349/TRANSNANOAVANSENS; Gobierno de España. CTQ2017-84309-C2-1-R; Gobierno de España. MAT2015-71879-P

Editor's Version

https://doi.org/10.1007/s00604-019-3386-9

Subjects

Cancer; DNA sensing; Fluorescent assay; Fluorescent carbon nanodots; Genetic diseases; Mutations; Rapid assay; Química

URI

http://hdl.handle.net/10486/689194

Note

This is a post-peer-review, pre-copyedit version of an article published in Microchimica Acta. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00604-019-3386-9

Rights

Abstract

The authors report on a fluorometric method for the rapid detection of BRCA1, CFRT and MRP3 gene mutations. These are associated with breast cancer, cystic fibrosis and autoimmune hepatitis diseases, respectively. Carbon nanodots with blue fluorescence (with excitation/emission maxima at 340/440 nm) were synthesized and characterized, and their interactions with DNA were investigated. Changes in the fluorescence intensity following interaction with ssDNA and dsDNA were used for specific DNA sequence of BRCA1, CFRT and MRP3 genes detection. The response to DNAs is linear up to 200 nM and the detection limit is 270 pM. The assay selectivity allows the detection of single gene mutations. Under optimum conditions, the assay can rapidly discriminate between wild type and mutated samples

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