Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations
Entidad
UAM. Departamento de Química Analítica y Análisis InstrumentalEditor
Springer VerlagFecha de edición
2019-05-01Cita
10.1007/s00604-019-3386-9
Microchimica Acta 186.5 (2019): 293
ISSN
1436-5073 (online); 0026-3672 (print)DOI
10.1007/s00604-019-3386-9Financiado por
CAM projects: TRANSNANOAVANSENS-CM (S2018/NMT-4349) and MAD2D-CM Program. MEIC projects: CTQ2017-84309-C2-1-R and MAT2015-71879-P. We thank the Confocal Microscopy and Flow Cytometry Services of CBMSOProyecto
Comunidad de Madrid. S2013/MIT‐3007/MAD2D; Comunidad de Madrid. S2018/NMT-4349/TRANSNANOAVANSENS; Gobierno de España. CTQ2017-84309-C2-1-R; Gobierno de España. MAT2015-71879-PVersión del editor
https://doi.org/10.1007/s00604-019-3386-9Materias
Cancer; DNA sensing; Fluorescent assay; Fluorescent carbon nanodots; Genetic diseases; Mutations; Rapid assay; QuímicaNota
This is a post-peer-review, pre-copyedit version of an article published in Microchimica Acta. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00604-019-3386-9Derechos
© 2019, Springer-Verlag GmbH Austria, part of Springer NatureResumen
The authors report on a fluorometric method for the rapid detection of BRCA1, CFRT and MRP3 gene mutations. These are associated with breast cancer, cystic fibrosis and autoimmune hepatitis diseases, respectively. Carbon nanodots with blue fluorescence (with excitation/emission maxima at 340/440 nm) were synthesized and characterized, and their interactions with DNA were investigated. Changes in the fluorescence intensity following interaction with ssDNA and dsDNA were used for specific DNA sequence of BRCA1, CFRT and MRP3 genes detection. The response to DNAs is linear up to 200 nM and the detection limit is 270 pM. The assay selectivity allows the detection of single gene mutations. Under optimum conditions, the assay can rapidly discriminate between wild type and mutated samples
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Google Scholar:García-Mendiola, Tania
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Elosegui, Cristina Garcia
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Bravo, Iria
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Pariente Alonso, Félix
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Jacobo-Martin, Alejandra
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Navio, Cristina
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Rodriguez, Isabel
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Wannemacher, Reinhold
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Lorenzo, Encarnación
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