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Fluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutations

dc.contributor.authorGarcía-Mendiola, Tania
dc.contributor.authorElosegui, Cristina Garcia
dc.contributor.authorBravo, Iria
dc.contributor.authorPariente Alonso, Félix 
dc.contributor.authorJacobo-Martin, Alejandra
dc.contributor.authorNavio, Cristina
dc.contributor.authorRodriguez, Isabel
dc.contributor.authorWannemacher, Reinhold
dc.contributor.authorLorenzo, Encarnación
dc.contributor.otherUAM. Departamento de Química Analítica y Análisis Instrumentales_ES
dc.date.accessioned2019-11-12T10:11:00Z
dc.date.available2019-11-12T10:11:00Z
dc.date.issued2019-05-01
dc.identifier.citationMicrochimica Acta 186.5 (2019): 293en_US
dc.identifier.issn1436-5073 (online)en_US
dc.identifier.issn0026-3672 (print)en_US
dc.identifier.urihttp://hdl.handle.net/10486/689194
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in Microchimica Acta. The final authenticated version is available online at: http://dx.doi.org/10.1007/s00604-019-3386-9en_US
dc.description.abstractThe authors report on a fluorometric method for the rapid detection of BRCA1, CFRT and MRP3 gene mutations. These are associated with breast cancer, cystic fibrosis and autoimmune hepatitis diseases, respectively. Carbon nanodots with blue fluorescence (with excitation/emission maxima at 340/440 nm) were synthesized and characterized, and their interactions with DNA were investigated. Changes in the fluorescence intensity following interaction with ssDNA and dsDNA were used for specific DNA sequence of BRCA1, CFRT and MRP3 genes detection. The response to DNAs is linear up to 200 nM and the detection limit is 270 pM. The assay selectivity allows the detection of single gene mutations. Under optimum conditions, the assay can rapidly discriminate between wild type and mutated samplesen_US
dc.description.sponsorshipCAM projects: TRANSNANOAVANSENS-CM (S2018/NMT-4349) and MAD2D-CM Program. MEIC projects: CTQ2017-84309-C2-1-R and MAT2015-71879-P. We thank the Confocal Microscopy and Flow Cytometry Services of CBMSOen_US
dc.format.extent17 pag.en_US
dc.format.mimetypeapplication/pdfen
dc.language.isoengen
dc.publisherSpringer Verlages_ES
dc.relation.ispartofMicrochimica Acta
dc.rights© 2019, Springer-Verlag GmbH Austria, part of Springer Natureen_US
dc.subject.otherCanceren_US
dc.subject.otherDNA sensingen_US
dc.subject.otherFluorescent assayen_US
dc.subject.otherFluorescent carbon nanodotsen_US
dc.subject.otherGenetic diseasesen_US
dc.subject.otherMutationsen_US
dc.subject.otherRapid assayen_US
dc.titleFluorescent C-NanoDots for rapid detection of BRCA1, CFTR and MRP3 gene mutationsen_US
dc.typearticleen
dc.subject.ecienciaQuímicaes_ES
dc.date.embargoend2020-05-01
dc.relation.publisherversionhttps://doi.org/10.1007/s00604-019-3386-9es_ES
dc.identifier.doi10.1007/s00604-019-3386-9es_ES
dc.identifier.publicationfirstpage293-1es_ES
dc.identifier.publicationissue5es_ES
dc.identifier.publicationlastpage293-8es_ES
dc.identifier.publicationvolume186es_ES
dc.relation.projectIDComunidad de Madrid. S2013/MIT‐3007/MAD2Des_ES
dc.relation.projectIDComunidad de Madrid. S2018/NMT-4349/TRANSNANOAVANSENSes_ES
dc.relation.projectIDGobierno de España. CTQ2017-84309-C2-1-Res_ES
dc.relation.projectIDGobierno de España. MAT2015-71879-Pes_ES
dc.type.versioninfo:eu-repo/semantics/acceptedVersionen
dc.rights.accessRightsopenAccessen
dc.authorUAMGarcía Mendiola, Tania (261838)
dc.authorUAMBravo Segura, Iria (265034)
dc.authorUAMPariente Alonso, Félix (259254)
dc.authorUAMLorenzo Abad, Encarnación (261505)
dc.facultadUAMFacultad de Ciencias
dc.institutoUAMInstituto de Investigación Avanzada en Ciencias Químicas (IAdChem)


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