Into the thermus mobilome: Presence, diversity and recent activities of insertion sequences across thermus spp.
Entity
Centro de Biología Molecular Severo Ochoa (CBM)Publisher
MDPI AGDate
2019-01-21Citation
10.3390/microorganisms7010025
Microorganisms 7.1 (2019): 25
ISSN
2076-2607DOI
10.3390/microorganisms7010025Funded by
This work was supported by grants BIO2016-77031-R from the Spanish Ministry of Economy and Competitiveness and grant nº 685474 from Horizon 2020 research and innovation program of the European Union. An institutional grant from Fundación Ramón Areces to the CBMSO is also acknowledgedProject
Gobierno de España. BIO2016-77031-R; info:eu-repo/grantAgreement/EC/H2020/685474/EU//METAFLUIDICSEditor's Version
https://doi.org/10.3390/microorganisms7010025Subjects
HGT; Insertion sequence; Mobilome; Thermophiles; Thermus; Transposases; Transposons; Biología y Biomedicina / BiologíaRights
© 2019 by the authors. Licensee MDPI, Basel, Switzerland.Abstract
A high level of transposon-mediated genome rearrangement is a common trait among microorganisms isolated from thermal environments, probably contributing to the extraordinary genomic plasticity and horizontal gene transfer (HGT) observed in these habitats. In this work, active and inactive insertion sequences (ISs) spanning the sequenced members of the genus Thermus were characterized, with special emphasis on three T. thermophilus strains: HB27, HB8, and NAR1. A large number of full ISs and fragments derived from different IS families were found, concentrating within megaplasmids present in most isolates. Potentially active ISs were identified through analysis of transposase integrity, and domestication-related transposition events of ISTth7 were identified in laboratory-adapted HB27 derivatives. Many partial copies of ISs appeared throughout the genome, which may serve as specific targets for homologous recombination contributing to genome rearrangement. Moreover, recruitment of IS1000 32 bp segments as spacers for CRISPR sequence was identified, pointing to the adaptability of these elements in the biology of these thermophiles. Further knowledge about the activity and functional diversity of ISs in this genus may contribute to the generation of engineered transposons as new genetic tools, and enrich our understanding of the outstanding plasticity shown by these thermophiles
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Google Scholar:Blesa, Alba
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Sánchez, Mercedes
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Sacristán-Horcajada, Eva
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de la Fuente, Sandra González
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Peiró, Ramón
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Berenguer Carlos, José
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