Tailored enzymatic synthesis of chitooligosaccharides with different deacetylation degrees and their anti-inflammatory activity
Entity
UAM. Departamento de Biología MolecularPublisher
MDPIAGDate
2019-05-01Citation
10.3390/catal9050405
Catalysts 9.5 (2019): 405
ISSN
2073-4344DOI
10.3390/catal9050405Funded by
This work was supported by the Fundación Ramón Areces (XIX Call of Research Grants in Life and Materials Sciences) and the Spanish Ministry of Economy and Competitiveness (Grants BIO2016-76601-C3-1-R and BIO2016-76601-C3-3-R). We also acknowledge the institutional grant from Ramon Areces Foundation to the Centre of Molecular Biology “Severo Ochoa”. The European Union’s Horizon 2020 program also financed this work (Blue Growth: Unlocking the potential of Seas and Oceans; grant agreement No. 634486; INMARE). We thank the support of the EU COST-Action CM1303 on Systems BiocatalysisProject
Gobierno de España. BIO2016-76601-C3-1-R; Gobierno de España. BIO2016-76601-C3-3-R; info:eu-repo/grantAgreement/EC/H2020/634486/EU//INMAREEditor's Version
https://doi.org/10.3390/catal9050405Subjects
Anti-inflammatory; Biocatalysis; Chitinases; Chitosan oligosaccharides; Chitosanases; Deacetylation degree; Glycosidases; Biología y Biomedicina / BiologíaRights
© 2019 by the authorsAbstract
By controlled hydrolysis of chitosan or chitin with different enzymes, three types of chitooligosaccharides (COS) with MW between 0.2 and 1.2 kDa were obtained: Fully deacetylated (fdCOS), partially acetylated (paCOS), and fully acetylated (faCOS). The chemical composition of the samples was analyzed by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD) and MALDI-TOF mass spectrometry. The synthesized fdCOS was basically formed by GlcN, (GlcN)2, (GlcN)3, and (GlcN)4. On the contrary, faCOS contained mostly GlcNAc, (GlcNAc)2 and (GlcNAc)3, while paCOS corresponded to a mixture of at least 11 oligosaccharides with different proportions of GlcNAc and GlcN. The anti-inflammatory activity of the three COS mixtures was studied by measuring their ability to reduce the level of TNF-α (tumor necrosis factor) in murine macrophages (RAW 264.7) after stimulation with a mixture of lipopolysaccharides (LPS). Only fdCOS and faCOS were able to significantly reduce the production of tumor necrosis factor (TNF)-α at 6 h after stimulation with lipopolysaccharides
Files in this item
Google Scholar:Santos-Moriano, P.
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Kidibule, P.
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Míguez, N.
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Fernández-Arrojo, L.
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Ballesteros, A. O.
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Fernández Lobato, María
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Plou, F. J.
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