Complete assembly of the Leishmania donovani (HU3 strain) genome and transcriptome annotation
EntityUAM. Departamento de Biología Molecular
PublisherNature Publishing Group
10.1038/s41598-019-42511-4Scientific Reports 2019.9 (2019): 6127
Funded byThis work was supported by grants (to B.A. and J.M.R.) from Proyecto del Ministerio de Economía, Industria y Competitividad SAF2017-86965-R (cofunded with FEDER funds), and by the Network of Tropical Diseases Research RICET (RD16/0027/0008) and FEDER. Institutional grants from the Fundación Ramón Areces and Banco de Santander to the CBMSO are also acknowledged
ProjectGobierno de España. SAF2017-86965-R; Gobierno de España. RD16/0027/0008
SubjectsParasite genomics; Transcriptomics; Biología y Biomedicina / Biología
Rights© 2019, The Author(s)
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
Leishmania donovani is a unicellular parasite that causes visceral leishmaniasis, a fatal disease in humans. In this study, a complete assembly of the genome of L. donovani is provided. Apart from being the first published genome of this strain (HU3), this constitutes the best assembly for an L. donovani genome attained to date. The use of a combination of sequencing platforms enabled to assemble, without any sequence gap, the 36 chromosomes for this species. Additionally, based on this assembly and using RNA-seq reads derived from poly-A + RNA, the transcriptome for this species, not yet available, was delineated. Alternative SL addition sites and heterogeneity in the poly-A addition sites were commonly observed for most of the genes. After a complete annotation of the transcriptome, 2,410 novel transcripts were defined. Additionally, the relative expression for all transcripts present in the promastigote stage was determined. Events of cis-splicing have been documented to occur during the maturation of the transcripts derived from genes LDHU3_07.0430 and LDHU3_29.3990. The complete genome assembly and the availability of the gene models (including annotation of untranslated regions) are important pieces to understand how differential gene expression occurs in this pathogen, and to decipher phenotypic peculiarities like tissue tropism, clinical disease, and drug susceptibility
Google Scholar:Camacho, Esther - González-de la Fuente, Sandra - Rastrojo, Alberto - Peiró-Pastor, Ramón - Solana, Jose Carlos - Tabera, Laura - Gamarro, Francisco - Carrasco-Ramiro, Fernando - Requena Rolania, José María - Aguado, Begoña
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