Sex differences in placental protein expression and efficiency in a rat model of fetal programming induced by maternal undernutrition
EntityUAM. Departamento de Fisiología
10.3390/ijms22010237Int J Mol Sci 22.1 (2020): 237
Funded byThis research was funded by the Ministerio de Ciencia, Innovación y Universidades (Spain), grant number RTI2018-097504-B-I00, cofinanced with FEDER funds and by the Faculty of Medicine, Khon Kaen University (Thailand), grant number KKU:0514.7.I.12-1948
ProjectGobierno de España. RTI2018-097504-B-I00
SubjectsCorticosterone; Maternal undernutrition; Oxidative stress; Placenta; Sex; VEGF; Medicina
Rights© 2020 by the authors. Licensee MDPI, Basel, Switzerland
Esta obra está bajo una Licencia Creative Commons Atribución 4.0 Internacional.
Fetal undernutrition programs cardiometabolic diseases, with higher susceptibility in males. The mechanisms implicated are not fully understood and may be related to sex differences in placental adaptation. To evaluate this hypothesis, we investigated placental oxidative balance, vascularization, glucocorticoid barrier, and fetal growth in rats exposed to 50% global nutrient re-striction from gestation day 11 (MUN, n = 8) and controls (n = 8). At gestation day 20 (G20), we analyzed maternal, placental, and fetal weights; oxidative damage, antioxidants, corticosterone, and PlGF (placental growth factor, spectrophotometry); and VEGF (vascular endothelial growth factor), 11β-HSD2, p22phox, XO, SOD1, SOD2, SOD3, catalase, and UCP2 expression (Western blot). Compared with controls, MUN dams exhibited lower weight and plasma proteins and higher corticosterone and catalase without oxidative damage. Control male fetuses were larger than female fetuses. MUN males had higher plasma corticosterone and were smaller than control males, but had similar weight than MUN females. MUN male placenta showed higher XO and lower 11β-HSD2, VEGF, SOD2, catalase, UCP2, and feto-placental ratio than controls. MUN females had similar feto-placental ratio and plasma corticosterone than controls. Female placenta expressed lower XO, 11β-HSD2, and SOD3; similar VEGF, SOD1, SOD2, and UCP2; and higher catalase than controls, being 11β-HSD2 and VEGF higher compared to MUN males. Male placenta has worse adaptation to un-dernutrition with lower efficiency, associated with oxidative disbalance and reduced vasculariza-tion and glucocorticoid barrier. Glucocorticoids and low nutrients may both contribute to programming in MUN males
Google Scholar:Phuthong, Sophida - Reyes-Hernández, Cynthia Guadalupe - Rodríguez-Rodríguez, Pilar - Ramiro-Cortijo, David - Gil-Ortega, Marta - González-Blázquez, Raquel - Carmen González, M. - de Pablo, Angel Luis López - Arribas Rodríguez, Silvia Magdalena
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