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dc.contributor.authorGarcía-Olmo, Dolores C.
dc.contributor.authorPeiró-Pastor, Ramón
dc.contributor.authorPicazo, María G.
dc.contributor.authorOlmedillas-López, Susana
dc.contributor.authorGarcía Arranz, Mariano Andrés 
dc.contributor.authorAguado, Begoña
dc.contributor.authorGarcía Olmo, Damián 
dc.contributor.otherUAM. Departamento de Cirugíaes_ES
dc.date.accessioned2021-04-23T14:17:04Z
dc.date.available2021-04-23T14:17:04Z
dc.date.issued2020-11-01
dc.identifier.citationTranslational Oncology 13.11 (2020): 100837en_US
dc.identifier.issn1936-5233 (es_ES
dc.identifier.urihttp://hdl.handle.net/10486/694834
dc.description.abstractDifferential presence of exons (DPE) is a method of interpretation of exome sequencing, which has been proposed to design a predictive algorithm with clinical value in patients with colorectal cancer (CRC). The goal of the present study was to examine the reproducibility in a rat model of metastatic colon cancer. DHD/K12-TRb cells were injected in syngenic immunocompetent BD-IX rats. Cells were from two stocks with low and normal metastatic potential, and injected into two separate groups of rats. Five to ten weeks after injection, blood samples were taken prior euthanasia and whole exome sequencing performed. Through DPE analysis, we identified a set of exons whose differential presence in plasma allowed us to compare both groups of tumor-bearing animals. A verification test was performed to confirm that the algorithm was able to classify extracted samples into their corresponding groups of origin. The highest mean probability was 0.8954. In conclusion, the DPE analysis in tumor-bearing animals was able to discriminate between different disease status, which fully supports previous results in CRC patients.en_US
dc.description.sponsorshipThis studywas funded by two grants from"Instituto de Salud Carlos III", Spain (FIS; refs. PS09/01815 and PI13/01924)en_US
dc.format.extent9 pag.es_ES
dc.format.mimetypeapplication/pdfen
dc.language.isoengen_US
dc.publisherElsevier Inc. on behalf of Neoplasia Press, Incen_US
dc.relation.ispartofTranslational Oncologyen_US
dc.rights© 2020 The authorsen_US
dc.subject.otherDifferential presence of exons (DPE)en_US
dc.subject.otherColorectal cancer (CRC).en_US
dc.subject.otherMetastatic colon cancer.en_US
dc.subject.otherAlgorithmen_US
dc.titleLiquid biopsy by NGS: Differential presence of exons (DPE) is related to metastatic potential in a colon-cancer model in the raten_US
dc.typearticleen
dc.subject.ecienciaMedicinaes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.tranon.2020.100837es_ES
dc.identifier.doi10.1016/j.tranon.2020.100837es_ES
dc.identifier.publicationfirstpage100837-1es_ES
dc.identifier.publicationissue11es_ES
dc.identifier.publicationlastpage100837-9es_ES
dc.identifier.publicationvolume13es_ES
dc.relation.projectIDGobierno de España. PS09/01815es_ES
dc.relation.projectIDGobierno de España. PI13/01924es_ES
dc.type.versioninfo:eu-repo/semantics/publishedVersionen
dc.rights.ccInstituto de Investigación Sanitaria Fundación Jiménez Díaz (ISS-FJD)es_ES
dc.rights.ccReconocimiento – NoComercial – SinObraDerivadaes_ES
dc.rights.accessRightsopenAccessen
dc.authorUAMGarcía Arranz, Mariano Andrés (262199)
dc.facultadUAMFacultad de Medicina
dc.institutoUAMInstituto de Investigación Sanitaria Fundación Jiménez Díaz (ISS-FJD)


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