Mañana, JUEVES, 24 DE ABRIL, el sistema se apagará debido a tareas habituales de mantenimiento a partir de las 9 de la mañana. Lamentamos las molestias.
Impact of long-term hfd intake on the peripheral and central igf system in male and female mice
Entity
UAM. Departamento de Pediatría; Instituto de Investigación Sanitaria Hospital Universitario de La Princesa (IIS-IP)Publisher
MDPI, Basel, Switzerland.Date
2020-11-13Citation
10.3390/metabo10110462
Metabolites 10.11 (2020): 462.
ISSN
2218-1989DOI
10.3390/metabo10110462Funded by
This research was funded by grants from the Spanish Ministry of Science and Innovation (BFU2017-82565-C21-R2 to J.A.C. and L.M.F.), Spanish Ministry of Education, Culture and Sports (university training grant PU13/00909 to A.F.-R.), Fondo de Investigación Sanitaria (PI1900166 to J.A.) and Fondos FEDER. Centro de Investigación Biomédica en Red Fisiopatología de Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III (J.A.)Project
Gobierno de España. BFU2017-82565-C21-R2; Gobierno de España. PU13/00909; Gobierno de España. PI1900166Editor's Version
https://doi.org/10.3390/metabo10110462Subjects
High-fat diet; IGF1; IGF2; IGFBP2; Neuropeptides; Obesity; Sex differences; MedicinaRights
© 2020 The authorsAbstract
The insulin-like growth factor (IGF) system is responsible for growth, but also affects metabolism and brain function throughout life. New IGF family members (i.e., pappalysins and stanniocalcins) control the availability/activity of IGFs and are implicated in growth. However, how diet and obesity modify this system has been poorly studied. We explored how intake of a high-fat diet (HFD) or commercial control diet (CCD) affects the IGF system in the circulation, visceral adipose tissue (VAT) and hypothalamus. Male and female C57/BL6J mice received HFD (60% fat, 5.1 kcal/g), CCD (10% fat, 3.7 kcal/g) or chow (3.1 % fat, 3.4 kcal/g) for 8 weeks. After 7 weeks of HFD intake, males had decreased glucose tolerance (p < 0.01) and at sacrifice increased plasma insulin (p < 0.05) and leptin (p < 0.01). Circulating free IGF1 (p < 0.001), total IGF1 (p < 0.001), IGF2 (p < 0.05) and IGFBP3 (p < 0.01) were higher after HFD in both sexes, with CCD increasing IGFBP2 in males (p < 0.001). In VAT, HFD reduced mRNA levels of IGF2 (p < 0.05), PAPP-A (p < 0.001) and stanniocalcin (STC)-1 (p < 0.001) in males. HFD increased hypothalamic IGF1 (p < 0.01), IGF2 (p < 0.05) and IGFBP5 (p < 0.01) mRNA levels, with these changes more apparent in females. Our results show that diet-induced changes in the IGF system are tissue-, sex-and diet-dependent.
Files in this item
Google Scholar:Guerra-Cantera, Santiago
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Frago Fernández, Laura María
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Jiménez-Hernaiz, María
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Ros Pérez, Purificación
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Freire-Regatillo, Alejandra
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Barrios, Vicente
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Argente Oliver, Jesús
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Chowen, Julie A.
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