Biological production of H2, CH4 and CO2 in the deep subsurface of the iberian pyrite belt
Entity
UAM. Departamento de Biología MolecularPublisher
WileyDate
2021-07-01Citation
10.1111/1462-2920.15561
Environmental Microbiology 23.7 (2021): 3913-3922
ISSN
1462-2912 (print); 1462-2920 (online)DOI
10.1111/1462-2920.15561Funded by
Authors thank all the IPBSL project team members for facilitating access to the samples. This work was supported by MICINN grant PID2019‐1048126GB‐I00. Thanks are due to A. I. Morato for her valuable technical assistanceProject
Gobierno de España. PID2019-1048126GB-I00Editor's Version
https://doi.org/10.1111/1462-2920.15561Subjects
Subsurface; Iberian pyrite belt; Gases; Biología y Biomedicina / BiologíaRights
© 2021 The AuthorsEsta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Abstract
Most of the terrestrial deep subsurfaces are oligotrophic environments in which some gases, mainly H2, CH4 and CO2, play an important role as energy and/or carbon sources. In this work, we assessed their biotic and abiotic origin in samples from subsurface hard-rock cores of the Iberian Pyrite Belt (IPB) at three different depths (414, 497 and 520 m). One set of samples was sterilized (abiotic control) and all samples were incubated under anaerobic conditions. Our results showed that H2, CH4 and CO2 remained low and constant in the sterilized controls while their levels were 4, 4.1 and 2.5 times higher respectively, in the unsterilized samples compared to the abiotic controls. The δ13CCH4-values measured in the samples (range −31.2 to −43.0 ‰) reveals carbon isotopic signatures that are within the range for biological methane production. Possible microorganisms responsible for the biotic production of the gases were assessed by CARD-FISH. The analysis of sequenced genomes of detected microorganisms within the subsurface of the IPB allowed to identify possible metabolic activities involved in H2 (Rhodoplanes, Shewanella and Desulfosporosinus), CH4 (Methanobacteriales) and CO2 production. The obtained results suggest that part of the H2, CH4 and CO2 detected in the deep subsurface has a biological origin
Files in this item
Google Scholar:Sanz, Jose L.
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Rodríguez Salas, Nuria
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Escudero, Cristina
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Carrizo, Daniel
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Amils Pibernat, Ricardo
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