Synchrotron Radiation-Fourier transformed infrared microspectroscopy (μSR-FTIR) reveals multiple metabolism alterations in microalgae induced by cadmium and mercury
EntityUAM. Departamento de Biología
10.1016/j.jhazmat.2021.126502Journal of Hazardous Materials 419 (2021): 126502
Funded byWork supported by the Spanish State Research Agency (AEI) (Spain) (projects AGL2014–53771-R and AGL2017–87591-R). The FTIR experiments were performed at MIRAS beamline at ALBA Synchrotron with the collaboration of ALBA staff and supported by travel grants for experiments No 2016091860 and 2017022112
ProjectGobierno de España. Gobierno de España. AGL2014–53771-R; Gobierno de España. Gobierno de España. AGL2017-87591-R
SubjectsBiomolecular alterations; Chlamydomonas; Metal toxicity; Oxidative stress; Synchrotron radiation FTIR; Biología y Biomedicina / Biología
Rights© 2021 The Authors
Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Toxic metals such as cadmium (Cd) and mercury (Hg) represent a threat to photosynthetic organisms of polluted aquatic ecosystems, and knowledge about mechanisms of toxicity is essential for appropriate assessment of environmental risks. We used Synchrotron Radiation-Fourier Transformed Infrared microspectroscopy (μSR-FTIR) to characterise major changes of biomolecules caused by Cd and Hg in the model green microalga Chlamydomonas reinhardtii. μSR-FTIR showed several metabolic alterations in different biochemical groups such as carbohydrates, proteins, and lipids in a time-dose dependent manner, with the strongest changes occurring at concentrations above 10 μM Cd and 15 μM Hg after short-term (24 h) treatments. This occurred in a context where metals triggered intracellular oxidative stress and chloroplast damage, along with autophagy induction by overexpressing AUTOPHAGY-RELATED PROTEIN 8 (ATG8). Thin layer chromatography analysis confirmed that toxic metals promoted remarkable changes in lipid profile, with higher degree of esterified fatty acid unsaturation as detected by gas chromatography coupled with mass spectrometry. Under Cd stress, there was specifically higher unsaturation of free fatty acids, while Hg led to stronger unsaturation in monogalactosyldiacylglycerol. μSR-FTIR spectroscopy proved as a valuable tool to identify biochemical alterations in microalgae, information that could be exploited to optimise approaches for metal decontamination
Google Scholar:Barón-Sola, Ángel - Toledo-Basantes, Margarita - Arana-Gandía, María - Martínez, Flor - Ortega Villasante, Cristina - Dučić, Tanja - Yousef, Ibraheem - Hernández, Luis E.
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