Optical detection of atherosclerosis at molecular level by optical coherence tomography: an in vitro study
Entity
UAM. Departamento de Biología; UAM. Departamento de Física Aplicada; UAM. Departamento de Física de MaterialesPublisher
ElsevierDate
2022-07-01Citation
10.1016/j.nano.2022.102556
Nanomedicine: Nanotechnology, Biology and Medicine 43 (2022): 102556
ISSN
1549-9634 (print)DOI
10.1016/j.nano.2022.102556Funded by
Ministerio de Ciencia e Innovación (PID2019-106211RB-I00); Instituto de Salud Carlos III (PI16/00812) and (PI19/00565); Comunidad Autónoma de Madrid (S2017/BMD-3867 RENIM-CM) co-financed by the European Structural and Investment Fund; Horizon 2020 FET Open grant agreement no. 801305 (NanoTBTech project); Fundación para la Investigación Biomédica del Hospital Universitario Ramón y Cajal project IMP18_38 (2018/0265); COST action CA1740; Chinese Academy of Sciences Pioneer “Hundred Talents Program” Young talents (Class C) (JH)Project
Gobierno de España. PID2019-106211RB-I00; Gobierno de España. PI16/00812; Gobierno de España. PI19/00565; info:eu-repo/grantAgrement/EC/H2020/801305Editor's Version
https://doi.org/10.1016/j.nano.2022.102556Subjects
Cardiovascular imaging; Gold nanoshells; Molecular imaging; Optical coherence tomography; Biología y Biomedicina / Biología; FísicaRights
© 2022 The AuthorsAbstract
There is an urgent need for contrast agents to detect the first inflammation stage of atherosclerosis by cardiovascular optical coherence tomography (CV-OCT), the imaging technique with the highest spatial resolution and sensitivity of those used during coronary interventions. Gold nanoshells (GNSs) provide the strongest signal by CV-OCT. GNSs are functionalized with the cLABL peptide that binds specifically to the ICAM-1 molecule upregulated in the first stage of atherosclerosis. Dark field microscopy and CV-OCT are used to evaluate the specific adhesion of these functionalized GNSs to activated endothelial cells. This adhesion is investigated under static and dynamic conditions, for shear stresses comparable to those of physiological conditions. An increase in the scattering signal given by the functionalized GNSs attached to activated cells is observed compared to non-activated cells. Thus, cLABL-functionalized GNSs behave as excellent contrast agents for CV-OCT and promise a novel strategy for clinical molecular imaging of atherosclerosis
Files in this item
Google Scholar:Muñoz Ortiz, Tamara
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Hu, Jie
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Sanz Rodríguez, Francisco
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Ortgies, Dirk H.
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Jaque García, Daniel
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Méndez González, Diego
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Aguilar, Río
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Alfonso Manterola, Fernando
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Rivero, Fernando
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Martín Rodríguez, Emma
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García Solé, José
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