Sensitive SARS-CoV-2 detection in wastewaters using a carbon nanodot-amplified electrochemiluminescence immunosensor
Entity
UAM. Departamento de Química Analítica y Análisis InstrumentalPublisher
ElsevierDate
2022-05-13Citation
10.1016/j.talanta.2022.123543
Talanta 247 (2022): 123543
ISSN
0039-9140 (print)DOI
10.1016/j.talanta.2022.123543Funded by
This work has been supported by the Spanish Ministerio de Ciencia e Innovacion (PID2020-116728RB-I00) and the Comunidad Autonoma ´ de Madrid (S2018/NMT-4349 TRANSNANOAVANSENS-CM Program, SI3/ PJI/2021-00341 CM Program and 2017-T1/BIO-5435 and 2021-5A/ BIO-20943 Talent Attraction Project)Project
Gobierno de España. PID2020-116728RB-I00Editor's Version
https://doi.org/10.1016/j.talanta.2022.123543Subjects
COVID-19; Electrochemiluminescence; Immunosensor; SARS-CoV-2 spike S1 protein; Wastewater; QuímicaRights
© 2022 The Authors
Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial-SinObraDerivada 4.0 Internacional.
Abstract
Given the great utility that having fast, efficient and cost-effective methods for the detection of SARS-CoV-2 in wastewater can have in controlling the pandemic caused by this virus, the development of new dependable and specific SARS-CoV-2 coronavirus sensing devices to be applied to wastewater is essential to promote public health interventions. Therefore, herein we propose a new method to detect SARS-CoV-2 in wastewater based on a carbon nanodots-amplified electrochemiluminescence immunosensor for the determination of the SARS-CoV-2 Spike S1 protein. For the construction of the immunosensor, N-rich carbon nanodots have been synthetized with a double function: to contribute as amplifiers of the electrochemiluminescent signal in presence of [Ru(bpy)3]2+ and as antibody supports by providing functional groups capable of covalently interacting with the SARS-CoV-2 Spike S1 antibody. The proposed ECL immunosensor has demonstrated a high specificity in presence of other virus-related proteins and responded linearly to SARS-CoV-2 Spike S1 concentration over a wide range with a limit of detection of 1.2 pg/mL. The immunosensor has an excellent stability and achieved the detection of SARS-CoV-2 Spike S1 in river and urban wastewater, which supplies a feasible and reliable sensing platform for early virus detection and therefore to protect the population. The detection of SARS-CoV-2 Spike S1 in urban wastewater can be used as a tool to measure the circulation of the virus in the population and to detect a possible resurgence of COVID-19
Files in this item
Google Scholar:Guerrero Esteban, Tamara
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Gutiérrez Sánchez, María Cristina
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Villa Manso, Ana M.
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Revenga Parra, Mónica
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Pariente Alonso, Félix
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Lorenzo Abad, Encarnación
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