Distinct patterns of gene expression during regeneration and asexual reproduction in the annelid Pristina leidyi
EntityUAM. Departamento de Biología; UAM. Departamento de Biología Molecular
10.1002/jez.b.23143Journal of Experimental Zoology Part B: Molecular and Developmental Evolution (2022): 1-16
ISSN1552-5007 (print); 1552-5015 (online)
Funded byH2020 Marie Sklodowska‐Curie Actions, Grant/Award Number: 841576; Comunidad de Madrid, Grant/Award Number: SI1/PJI/ 2019‐00532; Universidad Autónoma de Madrid, Grant/Award Number: 3931‐326F‐ 726DP6A38‐4F77; European Molecular Biology Organization, Grant/Award Number: ALTF‐217‐2018
SubjectsAnnelida; Differential gene expression; Paratomic fission; Pristina leidyi; Regeneration; Reproduction; Biología y Biomedicina / Biología
Rights© 2022 The Authors
Esta obra está bajo una licencia de Creative Commons Reconocimiento-NoComercial 4.0 Internacional.
Regeneration, the ability to replace lost body parts, is a widespread phenomenon in the animal kingdom often connected to asexual reproduction or fission, since the only difference between the two appears to be the stimulus that triggers them. Both developmental processes have largely been characterized; however, the molecular toolkit and genetic mechanisms underlying these events remain poorly unexplored. Annelids, in particular the oligochaete Pristina leidyi, provide a good model system to investigate these processes as they show diverse ways to regenerate, and can reproduce asexually through fission under laboratory conditions. Here, we used a comparative transcriptomics approach based on RNA-sequencing and differential gene expression analyses to understand the molecular mechanisms involved in anterior regeneration and asexual reproduction. We found 291 genes upregulated during anterior regeneration, including several regeneration-related genes previously reported in other annelids such as frizzled, paics, and vdra. On the other hand, during asexual reproduction, 130 genes were found upregulated, and unexpectedly, many of them were related to germline development during sexual reproduction. We also found important differences between anterior regeneration and asexual reproduction, with the latter showing a gene expression profile more similar to that of control individuals. Nevertheless, we identified 35 genes that were upregulated in both conditions, many of them related to cell pluripotency, stem cells, and cell proliferation. Overall, our results shed light on the molecular mechanisms that control anterior regeneration and asexual reproduction in annelids and reveal similarities with other animals, suggesting that the genetic machinery controlling these processes is conserved across metazoans
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